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Infection and Immunity, March 1999, p. 1508-1510, Vol. 67, No. 3
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Pseudomonas aeruginosa Exoenzyme S Is a Biglutamic Acid ADP-Ribosyltransferase

Jay Radke, Kristin J. Pederson, and Joseph T. Barbieri*

Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226

Received 26 October 1998/Returned for modification 23 November 1998/Accepted 8 December 1998

Kinetic analysis of two mutations within Pseudomonas aeruginosa exoenzyme S (ExoS) showed that a E379D mutation inhibited expression of ADP-ribosyltransferase activity but had little effect on the expression of NAD glycohydrolase activity while a E381D mutation inhibited expression of both activities. These data identify ExoS as a biglutamic acid ADP-ribosyltransferase, where E381 is the catalytic residue and E379 contributes to the transfer of ADP-ribose to the target protein.

* Corresponding author. Mailing address: Medical College of Wisconsin, Microbiology and Molecular Genetics, 8701 Watertown Plk. Rd., Milwaukee, WI 53226. Phone: (414) 456-8412. Fax: (414) 456-6535. E-mail: toxin{at}mcw.edu.

Infection and Immunity, March 1999, p. 1508-1510, Vol. 67, No. 3
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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