IAI FigSearch
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Andrews, G. P.
Right arrow Articles by Friedlander, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Andrews, G. P.
Right arrow Articles by Friedlander, A. M.

 Previous Article

Infection and Immunity, March 1999, p. 1533-1537, Vol. 67, No. 3
0019-9567/99

Protective Efficacy of Recombinant Yersinia Outer Proteins against Bubonic Plague Caused by Encapsulated and Nonencapsulated Yersinia pestis

G. P. Andrews,* S. T. Strachan, G. E. Benner, A. K. Sample, G. W. Anderson Jr.,dagger J. J. Adamovicz, S. L. Welkos, J. K. Pullen, and A. M. Friedlander*

Division of Bacteriology, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702-5011

Received 8 September 1998/Returned for modification 5 October 1998/Accepted 21 December 1998

To evaluate the role of Yersinia outer proteins (Yops) in conferring protective immunity against plague, six yop loci from Yersinia pestis were individually amplified by PCR, cloned, and expressed in Escherichia coli. The recombinant proteins were purified and injected into mice. Most Yop-vaccinated animals succumbed to infection with either wild-type encapsulated Y. pestis or a virulent, nonencapsulated isogenic variant. Vaccination with YpkA significantly prolonged mean survival time but did not increase overall survival of mice infected with the nonencapsulated strain. The only significant protection against death was observed in YopD-vaccinated mice challenged with the nonencapsulated strain.

* Corresponding author. Mailing address for G. P. Andrews: Department of Pathology & Area Laboratory Services, Landstuhl Regional Medical Center, D-66849 Landstuhl, Germany. Mailing address for A. M. Friedlander: Division of Bacteriology, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011. Phone: (301)-619-7341. Fax: (301)-619-2152. E-mail: friedlan{at}ncifcrf.gov.

dagger Present address: Southern Research Institute, Frederick, MD 21701-4756.

Infection and Immunity, March 1999, p. 1533-1537, Vol. 67, No. 3
0019-9567/99


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. J. Virol. Eukaryot. Cell
Microbiol. Mol. Biol. Rev. Clin. Vaccine Immunol. All ASM Journals

Copyright © 1999 by the American Society for Microbiology. All rights reserved.