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Infection and Immunity, April 1999, p. 1585-1592, Vol. 67, No. 4
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Isolation of Enterococcus faecalis Clinical Isolates That Efficiently Adhere to Human Bladder Carcinoma T24 Cells and Inhibition of Adhesion by Fibronectin and Trypsin Treatment

Akihiko Shiono,dagger and Yasuyoshi Ike*

Department of Microbiology and Laboratory of Bacterial Drug Resistance, Gunma University School of Medicine, Maebashi, Gunma, Japan

Received 15 June 1998/Returned for modification 12 August 1998/Accepted 17 December 1998

The adherence of Enterococcus faecalis strains to human T24 cells was examined by scanning electron microscopy. Five highly adhesive strains were identified from 30 strains isolated from the urine of patients with urinary tract infections. No efficiently adhesive strains were found among the 30 strains isolated from the feces of healthy students. The five isolated strains also adhered efficiently to human bladder epithelial cells. Analysis of restriction endonuclease-digested plasmid DNAs and chromosome DNAs showed that the five strains were different strains isolated from different patients. The adhesiveness of these strains was inhibited by treatment with fibronectin or trypsin, implying that a specific protein (adhesin) on the bacterial cell surface mediates adherence to fibronectin on the host cell surfaces, and the adhesin differs from the reported adhesins.

* Corresponding author. Mailing address: Department of Microbiology, Gunma University School of Medicine, Maebashi, Gunma 371-8511, Japan. Phone: 81-27-220-7990. Fax: 81-27-220-7996. E-mail: yasuike{at}sb.gunma-u.ac.jp.

dagger Present address: Department of Urology, Gunma University School of Medicine, Gunma, Japan.

Infection and Immunity, April 1999, p. 1585-1592, Vol. 67, No. 4
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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