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Infection and Immunity, April 1999, p. 1798-1805, Vol. 67, No. 4
Laboratoire de Bactériologie-Virologie
UMR CNRS 75-65,1 Laboratoire d'Anatomie
Pathologique,2 EP CNRS
616,3 and Laboratoire de Microscopie
Electronique,
Received 19 August 1998/Returned for modification 28 October
1998/Accepted 6 January 1999
In vitro or animal models have been used to investigate the
pathogenesis of Helicobacter pylori infection. However,
extrapolation to humans of results obtained with these heterologous
models remains difficult. We have developed a new model for the study
of H. pylori infection that uses human entire embryonic
stomachs engrafted in nude mice. At 80 days after implantation, 22 of
these xenografts, which exhibited a mature gastric epithelium, were
inoculated with 107 to 108 CFU of either
H. pylori LB1, a freshly isolated H. pylori
strain (n = 12), or H. pylori ATCC 49503 (n = 10). After 12-week examination, H. pylori LB1 persistently colonized the antrum of all inoculated grafts, as assessed by culture (mucus and mucosa), immunohistochemistry (mucosa), and a rapid urease test (mucus). H. pylori ATCC
49503, either before or after in vivo passage, permitted only a
transient 2-week colonization in one of the five inoculated grafts in
both groups. Colonization was always associated with an increase of gastric juice pH. A mild neutrophil infiltration of the gastric mucosa
was noted solely in infected grafts. Transmission electron microscopy
showed adherence of H. pylori organisms to epithelial cell
surface. In six animals, intracytoplasmic location of this bacterium
was observed in the antrum or the fundus. These results allow us to
propose this model as a new ex vivo model for the study of specific
H. pylori-gastric cell interactions.
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Human Embryonic Gastric Xenografts in Nude Mice: a
New Model of Helicobacter pylori Infection
*
Corresponding author. Mailing address: Laboratoire de
Bactériologie, Hôpital Central, 29 Avenue du Maréchal
de Lattre de Tassigny, 54035 Nancy Cedex, France. Phone: (33) 3 83 85 21 96. Fax: (33) 3 83 85 26 73. E-mail:
a.lozniewski{at}chu-nancy.fr.
Infection and Immunity, April 1999, p. 1798-1805, Vol. 67, No. 4
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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