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Infection and Immunity, April 1999, p. 1922-1928, Vol. 67, No. 4
0019-9567/99/$04.00+0

Immune Response to Yersinia Outer Proteins and Other Yersinia pestis Antigens after Experimental Plague Infection in Mice

Gretchen E. Benner, Gerard P. Andrews,dagger W. Russell Byrne, Susan D. Strachan, Allen K. Sample,Dagger David G. Heath,dagger and Arthur M. Friedlander*

Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, Frederick, Maryland 21702-5011

Received 26 May 1998/Returned for modification 13 August 1998/Accepted 12 January 1999

There is limited information concerning the nature and extent of the immune response to the virulence determinants of Yersinia pestis during the course of plague infection. In this study, we evaluated the humoral immune response of mice that survived lethal Y. pestis aerosol challenge after antibiotic treatment. Such a model may replicate the clinical situation in humans and indicate which virulence determinants are expressed in vivo. Immunoglobulin G enzyme-linked immunosorbent assay and immunoblotting were performed by using purified, recombinant antigens including F1, V antigen, YpkA, YopH, YopM, YopB, YopD, YopN, YopE, YopK, plasminogen activator protease (Pla), and pH 6 antigen as well as purified lipopolysaccharide. The major antigens recognized by murine convalescent sera were F1, V antigen, YopH, YopM, YopD, and Pla. Early treatment with antibiotics tended to reduce the immune response and differences between antibiotic treatment regimens were noted. These results may indicate that only some virulence factors are expressed and/or immunogenic during infection. This information may prove useful for selecting potential vaccine candidates and for developing improved serologic diagnostic assays.

* Corresponding author. Mailing address: Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, 1425 Porter St., Frederick, MD 21702-5011. Phone: (301) 619-7341. Fax: (301) 619-2152. E-mail: friedlan{at}ncifcrf.gov.

dagger Present address: Department of Pathology & Area Lab Services, Landstuhl Regional Medical Center, Landstuhl, Germany.

Dagger Present address: IDEXX Laboratories, Inc., Westbrook, ME 04092.

Infection and Immunity, April 1999, p. 1922-1928, Vol. 67, No. 4
0019-9567/99/$04.00+0


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