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Infection and Immunity, April 1999, p. 1962-1966, Vol. 67, No. 4
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Vaccination and Protection of Pigs against Pleuropneumonia with a Vaccine Strain of Actinobacillus pleuropneumoniae Produced by Site-Specific Mutagenesis of the ApxII Operon

C. T. Prideaux,* C. Lenghaus, J. Krywult, and A. L. M. Hodgson

Animal Health Laboratory, CSIRO Division of Animal Health, Geelong, Victoria 3120, Australia

Received 16 April 1998/Returned for modification 6 July 1998/Accepted 26 January 1999

The production of toxin (Apx)-neutralizing antibodies during infection plays a major role in the induction of protective immunity to Actinobacillus pleuropneumoniae reinfection. In the present study, the gene encoding the ApxII-activating protein, apxIIC, was insertionally inactivated on the chromosome of a serovar 7 strain, HS93. Expression of the structural toxin, ApxIIA, and of the two genes required for its secretion, apxIB and apxID, still occurs in this strain. The resulting mutant strain, HS93C- Ampr, was found to secrete the unactivated toxin. Pigs vaccinated with live HS93C- Ampr via the intranasal route were protected against a cross-serovar challenge with a virulent serovar 1 strain of A. pleuropneumoniae. This is the first reported vaccine strain of A. pleuropneumoniae which can be delivered live to pigs and offers cross-serovar protection against porcine pleuropneumonia.

* Corresponding author. CSIRO, Division of Animal Health, Animal Health Laboratory, Private Bag No. 24, Geelong, Victoria 3120, Australia. Phone: 61-3-5227 5000. Fax: 61-3-5227 5531. E-mail: Christopher.Prideaux{at}dah.csiro.au.

Infection and Immunity, April 1999, p. 1962-1966, Vol. 67, No. 4
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.