Levels of Antibody to Conserved Parts of Plasmodium falciparum Merozoite Surface Protein 1 in Ghanaian Children Are Not Associated with Protection from Clinical Malaria

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Infection and Immunity, May 1999, p. 2131-2137, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Levels of Antibody to Conserved Parts of Plasmodium falciparum Merozoite Surface Protein 1 in Ghanaian Children Are Not Associated with Protection from Clinical Malaria

Daniel Dodoo,¹^,²^,^* Thor G. Theander,² Jorgen A. L. Kurtzhals,¹^,² Kojo Koram,¹ Eleanor Riley,³ Bartholomew D. Akanmori,¹ Francis K. Nkrumah,¹ and Lars Hviid²

Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana¹; Centre for Medical Parasitology, Department of Infectious Diseases, Copenhagen University Hospital (Rigshospitalet), and Institute of Medical Microbiology and Immunology, University of Copenhagen, Copenhagen, Denmark²; and Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom³

Received 11 December 1998/Returned for modification 21 January 1999/Accepted 12 February 1999

The 19-kDa conserved C-terminal part of the Plasmodium falciparum merozoite surface protein 1 (PfMSP1₁₉) is a malaria vaccinecandidate antigen, and human antibody responses to PfMSP1₁₉ havebeen associated with protection against clinical malaria. In thislongitudinal study carried out in an area of stable but seasonalmalaria transmission with an estimated parasite inoculation ofabout 20 infective bites/year, we monitored 266 3- to 15-year-oldGhanaian children clinically and parasitologically over a periodof 18 months. Blood samples were collected at the beginning ofthe study before the major malaria season in April and after theseason in November. Using enzyme-linked immunosorbent assay, wemeasured antibody responses to recombinant gluthathione S-transferase-PfMSP1₁₉fusion proteins corresponding to the Wellcome and MAD20 allelicvariants in these samples. Prevalence of antibodies recognizingthe Wellcome 19 construct containing both epidermal growth factor(EGF)-like motifs in Wellcome type PfMSP1₁₉ was about 30%. Prevalenceof antibodies to constructs containing only the first EGF domainfrom either Wellcome or MAD20 type PfMSP1₁₉ was about 15%, whereasantibodies recognizing a construct containing only the secondEGF domain of MAD20 type PfMSP1₁₉ was found in only about 4% ofthe donors. Neither the prevalence nor the levels of any of theantibody specificities varied significantly with season, age,or sex. Significantly, and in contrast to previous reports fromother parts of West Africa, we found no evidence of an associationbetween antibody responses to PfMSP1₁₉ and clinical protectionagainstmalaria.

^* Corresponding author. Mailing address: Department of Infectious Diseases M7641, Rigshospitalet, Tagensvej 20, 2200 CopenhagenN, Denmark. Phone: 45 35 45 73 75. Fax: 45 35 45 76 44. E-mail:ddcmp{at}rh.dk.

Infection and Immunity, May 1999, p. 2131-2137, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

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