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Infection and Immunity, May 1999, p. 2193-2200, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Structural and Antigenic Properties of Merozoite Surface Protein 4 of Plasmodium falciparum

Lina Wang,1 Casilda G. Black,1 Vikki M. Marshall,2 and Ross L. Coppel1,*

Department of Microbiology, Monash University, Clayton, Victoria, 3168,1 and The Walter and Eliza Hall Institute of Medical Research, Victoria 3050,2 Australia

Received 31 August 1998/Returned for modification 11 November 1998/Accepted 8 February 1999

Merozoite surface protein 4 (MSP4) of Plasmodium falciparum is a glycosylphosphatidylinositol-anchored integral membrane protein of 272 residues that possesses a single epidermal growth factor (EGF)-like domain near the carboxyl terminus. We have expressed both full-length MSP4 and a number of fragments in Escherichia coli and have used these recombinant proteins to raise experimental antisera. All recombinant proteins elicited specific antibodies that reacted with parasite-derived MSP4 by immunoblotting. Antibody reactivity was highly dependent on the protein conformation. For example, reduction and alkylation of MSP4 almost completely abolished the reactivity of several antibody preparations, including specificities directed to regions of the protein that do not contain cysteine residues and are far removed from the cysteine-containing EGF-like domain. This indicated the presence of conformation-dependent epitopes in MSP4 and demonstrated that proper folding of the EGF-like domain influenced the antigenicity of the entire molecule. The recombinant proteins were used to map epitopes recognized by individuals living in areas where malaria is endemic, and at least four distinct regions are naturally antigenic during infection. Binding of human antibodies to the EGF-like domain was essentially abrogated after reduction of the recombinant protein, indicating the recognition of conformational epitopes by the human immune responses. This observation led us to examine the importance of conformation dependence in responses to other integral membrane proteins of asexual stages. We analyzed the natural immune responses to a subset of these antigens and demonstrated that there is diminished reactivity to several antigens after reduction. These studies demonstrate the importance of reduction-sensitive structures in the maintenance of the antigenicity of several asexual-stage antigens and in particular the importance of the EGF-like domain in the antigenicity of MSP4.

* Corresponding author. Mailing address: Department of Microbiology, Monash University, Clayton, 3168, Victoria, Australia. Phone: 61 3 9905 4822. Fax: 61 3 9905 4811. E-mail: ross.coppel{at}med.monash.edu.au.

Infection and Immunity, May 1999, p. 2193-2200, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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