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Infection and Immunity, May 1999, p. 2241-2249, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Bovine gamma delta T-Cell Responses to the Intracellular Protozoan Parasite Theileria parvadagger

Claudia A. Daubenberger,1,* Evans L. N. Taracha,1 Laima Gaidulis,1,Dagger William C. Davis,2 and Declan J. McKeever1

International Livestock Research Institute (ILRI), Nairobi, Kenya,1 and Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington2

Received 26 June 1998/Returned for modification 13 August 1998/Accepted 26 February 1999

T cells bearing the gamma delta antigen receptor (gamma delta T cells) can constitute up to 50% of T cells in the peripheral blood and lymphoid organs of young cattle. We present data showing that gamma delta T cells are involved in immune responses against Theileria parva. gamma delta T cells isolated from peripheral blood mononuclear cells (PBMC) of T. parva-naive and -immune cattle proliferated in the presence of fixed or unfixed autologous T. parva-infected lymphoblasts (TpL) and heat-stressed concanavalin A (ConA)-induced blasts (ConA blasts) but not untreated ConA blasts. The specificity of response was further evaluated with a panel of gamma delta T-cell lines and clones. T-cell reactivity was blocked by GB21A, a monoclonal antibody (MAb) specific for the gamma delta T-cell receptor, but not by MAbs specific for class I and class II major histocompatibility complex (MHC) molecules. In addition, TpL but not ConA blasts from a variety of MHC-mismatched animals induced proliferation of the gamma delta T-cell lines and clones. These gamma delta T cells were found to respond to TpL infected with several different parasite stocks and failed to recognize TpL after elimination of the parasite by the theilericidal drug BW 720C. Assays for cytotoxic activity of gamma delta T cells sorted from bulk cultures of immune PBMC restimulated several times with autologous TpL demonstrated that effector cells whose specificity is similar to that of proliferating cells are generated. These results suggest that bovine gamma delta T cells are activated by and lyse T. parva-infected cells by recognizing conserved parasite-induced or parasite-derived antigens in an MHC-unrestricted fashion.

* Corresponding author. Present address: Swiss Tropical Institute, Postfach, CH 4002 Basel, Switzerland. Phone: 41 61 284 8236. Fax: 41 61 271 8654. E-mail: Daubenberger{at}ubaclu.unibas.ch.

dagger This is ILRI publication no. 98026.

Dagger Present address: City of Hope National Medical Center, Duarte, CA 91010.

Infection and Immunity, May 1999, p. 2241-2249, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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