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Infection and Immunity, May 1999, p. 2497-2502, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Expression of Interleukin-6 in the Cornea in Response to Infection with Different Strains of Pseudomonas aeruginosa

Nerida Cole,1,2,3 Shisan Bao,3 Mark Willcox,1,2,* and Alan J. Husband3

Cooperative Research Centre for Eye Research and Technology1 and Corneal and Contact Lens Research Unit,2 School of Optometry, University of New South Wales, Sydney, New South Wales 2052, and Department of Anatomy and Pathology, University of Sydney, Sydney, New South Wales 2006,3 Australia

Received 24 June 1998/Returned for modification 23 September 1998/Accepted 29 January 1999

Strains of Pseudomonas aeruginosa causing keratitis can be either cytotoxic (6206) or invasive (6294), while a strain (Paer1) causing contact lens-induced acute red eye has been shown to be neither. In situ hybridization was used to examine the location and identity of cells expressing interleukin-6 (IL-6) mRNA in the murine cornea and changes in expression in response to infection with different strains of P. aeruginosa. The number of IL-6-positive cells was determined by image analysis. IL-6 protein levels were measured by an enzyme-linked immunosorbent assay. BALB/c mice were challenged by use of the wounded-cornea model with P. aeruginosa 6294, 6206, or Paer1 (2 × 106 CFU). At time intervals up to 24 h, postchallenge corneal tissue was probed for IL-6 mRNA. IL-6 mRNA expression was rapidly elevated in the epithelium in response to strains 6294 and 6206. At the conclusion of the experiments, infiltrating inflammatory cells also stained positively for IL-6 mRNA. In contrast, corneas challenged with strain Paer1 showed significant upregulation of IL-6 mRNA only at 4 h postchallenge. Three distinct patterns of IL-6 mRNA expression in the mouse cornea occur in response to these three ocular isolates of P. aeruginosa. The data obtained for mRNA expression in the cornea for all three strains of P. aeruginosa correlated well with IL-6 protein analysis of whole-eye homogenates. Differences in the cytokine responses to these strains correlate with differences in the pathology associated with each strain and may offer an opportunity to develop strategies for the improved management of ocular inflammation.

* Corresponding author. Mailing address: CRCERT and CCLRU, School of Optometry, University of New South Wales, Sydney, New South Wales 2052, Australia. Phone: 61 2 9385 0205. Fax: 61 2 9385 0202. E-mail: m.willcox{at}cclru.unsw.edu.au.

Infection and Immunity, May 1999, p. 2497-2502, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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