This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lehmann, A. K.
Right arrow Articles by Guttormsen, H.-K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lehmann, A. K.
Right arrow Articles by Guttormsen, H.-K.

 Previous Article  |  Next Article 

Infection and Immunity, May 1999, p. 2552-2560, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Human Opsonins Induced during Meningococcal Disease Recognize Outer Membrane Proteins PorA and PorB

A. K. Lehmann,1,* A. Halstensen,1 I. S. Aaberge,2 J. Holst,2 T. E. Michaelsen,2,3 S. Sørnes,1 L. M. Wetzler,4 and H.-K. Guttormsen5

Medical Department B, University of Bergen, Bergen,1 and Department of Vaccinology, National Institute of Public Health,2 and Institute of Pharmacy, Department of Pharmacognosy, University of Oslo,3 Oslo, Norway, and Maxwell Finland Laboratory for Infectious Diseases, Boston Medical Center, Boston University School of Medicine,4 and Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School,5 Boston, Massachusetts

Received 13 October 1998/Returned for modification 24 November 1998/Accepted 20 January 1999

Human opsonins directed against specific meningococcal outer membrane structures in sera obtained during meningococcal disease were quantified with a recently developed antigen-specific, opsonin-dependent phagocytosis and oxidative burst assay. Outer membrane vesicles (OMVs) and PorA (class 1) and PorB (class 3) proteins purified from mutants of the same strain (44/76; B:15:P1.7.16) were adsorbed to fluorescent beads, opsonized with acute- and convalescent-phase sera from 40 patients with meningococcal disease, and exposed to human leukocytes. Flow cytometric quantitation of the resulting leukocyte phagocytosis products (PPs) demonstrated that disease-induced serum opsonins recognized meningococcal OMV components and both porins. The PPPorA and PPPorB values induced by convalescent-phase sera correlated positively with the PPOMV values. However, the PPPorB values were higher than the PPPorA values in convalescent-phase sera (medians [ranges] of 754 [17 to 1,057] and 107 [4 to 458], respectively) (P < 0.0001) and correlated positively with higher levels of immunoglobulin G against PorB than against PorA as evaluated by enzyme-linked immunosorbent assay. Extensive individual variations in the anti-OMV and antiporin serum opsonic activities between patients infected by serotypes and serosubtypes homologous and heterologous to the target antigens were observed. Simultaneously measured oxidative burst activity correlated with the opsonophagocytosis, an indication that both of these important steps in the in vitro phagocytic elimination of meningococci are initiated by opsonins directed against OMV components, including PorA and PorB. In conclusion, human patient opsonins against meningococcal OMV components and in particular PorB epitopes were identified by this new method, which might facilitate selection of opsonin-inducing meningococcal antigens for inclusion in future vaccines.

* Corresponding author. Mailing address: Medical Department B, University of Bergen, Haukeland Hospital, N-5021 Bergen, Norway. Phone: 47 55 97 50 00. Fax: 47 55 97 29 50. E-mail: Anne.Lehmann{at}medb.uib.no.

Infection and Immunity, May 1999, p. 2552-2560, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Schultz, H., Hume, J., Zhang, D. S., Gioannini, T. L., Weiss, J. P. (2007). A Novel Role for the Bactericidal/Permeability Increasing Protein in Interactions of Gram-Negative Bacterial Outer Membrane Blebs with Dendritic Cells. J. Immunol. 179: 2477-2484 [Abstract] [Full Text]  
  • Toropainen, M., Saarinen, L., Vidarsson, G., Kayhty, H. (2006). Protection by Meningococcal Outer Membrane Protein PorA-Specific Antibodies and a Serogroup B Capsular Polysaccharide-Specific Antibody in Complement-Sufficient and C6-Deficient Infant Rats.. Infect. Immun. 74: 2803-2808 [Abstract] [Full Text]  
  • Domanski, P. J., Patel, P. R., Bayer, A. S., Zhang, L., Hall, A. E., Syribeys, P. J., Gorovits, E. L., Bryant, D., Vernachio, J. H., Hutchins, J. T., Patti, J. M. (2005). Characterization of a Humanized Monoclonal Antibody Recognizing Clumping Factor A Expressed by Staphylococcus aureus. Infect. Immun. 73: 5229-5232 [Abstract] [Full Text]  
  • Law, D. K. S., Henderson, A. M., Tsang, R. S. W. (2004). DNA Sequence Analysis of the PorB Protein of Nonserotypeable Serogroup C ET-15 Meningococci Suggests a Potential Mutational Hot Spot on Their Serotype Antigens. J. Clin. Microbiol. 42: 2718-2723 [Abstract] [Full Text]  
  • Newcombe, J., Eales-Reynolds, L.-J., Wootton, L., Gorringe, A. R., Funnell, S. G. P., Taylor, S. C., McFadden, J. J. (2004). Infection with an Avirulent phoP Mutant of Neisseria meningitidis Confers Broad Cross-Reactive Immunity. Infect. Immun. 72: 338-344 [Abstract] [Full Text]  
  • Vernachio, J., Bayer, A. S., Le, T., Chai, Y.-L., Prater, B., Schneider, A., Ames, B., Syribeys, P., Robbins, J., Patti, J. M. (2003). Anti-Clumping Factor A Immunoglobulin Reduces the Duration of Methicillin-Resistant Staphylococcus aureus Bacteremia in an Experimental Model of Infective Endocarditis. Antimicrob. Agents Chemother. 47: 3400-3406 [Abstract] [Full Text]  
  • Wright, J. C., Williams, J. N., Christodoulides, M., Heckels, J. E. (2002). Immunization with the Recombinant PorB Outer Membrane Protein Induces a Bactericidal Immune Response against Neisseria meningitidis. Infect. Immun. 70: 4028-4034 [Abstract] [Full Text]  
  • Bhasin, N., Ho, Y., Wetzler, L. M. (2001). Neisseria meningitidis Lipopolysaccharide Modulates the Specific Humoral Immune Response to Neisserial Porins but Has No Effect on Porin-Induced Upregulation of Costimulatory Ligand B7-2. Infect. Immun. 69: 5031-5036 [Abstract] [Full Text]  
  • Vidarsson, G., van der Pol, W.-L., van den Elsen, J. M. H., Vile, H., Jansen, M., Duijs, J., Morton, H. C., Boel, E., Daha, M. R., Corthesy, B., van de Winkel, J. G. J. (2001). Activity of Human IgG and IgA Subclasses in Immune Defense Against Neisseria meningitidis Serogroup B. J. Immunol. 166: 6250-6256 [Abstract] [Full Text]  
  • Wang, J., Jarvis, G. A., Achtman, M., Rosenqvist, E., Michaelsen, T. E., Aase, A., Griffiss, J. M. (2000). Functional Activities and Immunoglobulin Variable Regions of Human and Murine Monoclonal Antibodies Specific for the P1.7 PorA Protein Loop of Neisseria meningitidis. Infect. Immun. 68: 1871-1878 [Abstract] [Full Text]  
  • Guy, B., Testart, C., Gimenez, S., Sanchez, V., Lheritier, P., Rossin, D., Mignon, M., Danve, B., Trannoy, E. (2000). Comparison of Polymorphonuclear Cells from Healthy Donors and Differentiated HL-60 Cells as Phagocytes in an Opsonophagocytic Assay Using Antigen-Coated Fluorescent Beads. CVI 7: 314-317 [Abstract] [Full Text]  
  • Lehmann, A. K., Gorringe, A. R., Reddin, K. M., West, K., Smith, I., Halstensen, A. (1999). Human Opsonins Induced during Meningococcal Disease Recognize Transferrin Binding Protein Complexes. Infect. Immun. 67: 6526-6532 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»