IAI FigSearch
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sultan, A. A.
Right arrow Articles by Ménard, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sultan, A. A.
Right arrow Articles by Ménard, R.

 Previous Article  |  Next Article 

Infection and Immunity, May 1999, p. 2602-2606, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Green Fluorescent Protein as a Marker in Plasmodium berghei Transformation

Ali A. Sultan,1 Vandana Thathy,2 Victor Nussenzweig,1 and Robert Ménard1,2,*

Michael Heidelberger Division of Immunology, Department of Pathology, Kaplan Cancer Center,1 and Department of Medical and Molecular Parasitology,2 New York University Medical Center, New York, New York 10016

Received 14 December 1998/Returned for modification 18 January 1999/Accepted 8 February 1999

We present a new marker that confers both resistance to pyrimethamine and green fluorescent protein-based fluorescence on the malarial parasite Plasmodium berghei. A single copy of the cassette integrated into the genome is sufficient to direct fluorescence in parasites throughout the life cycle, in both its mosquito and vertebrate hosts. Erythrocyte stages of the parasite that express the marker can be sorted from control parasites by flow cytometry. Pyrimethamine pressure is not necessary for maintaining the cassette in transformed parasites during their sporogonic cycle in mosquitoes, including when it is borne by a plasmid. This tool should thus prove useful in molecular studies of P. berghei, both for generating parasite variants and monitoring their behavior.

* Corresponding author. Mailing address: Department of Pathology, NYU School of Medicine, 550 First Ave., New York, NY 10016. Phone: (212) 263-7870. Fax: (212) 263-8179. E-mail: menarr01{at}mcrcr6.med.nyu.edu.

Infection and Immunity, May 1999, p. 2602-2606, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. J. Virol. Eukaryot. Cell
Microbiol. Mol. Biol. Rev. Clin. Vaccine Immunol. All ASM Journals

Copyright © 1999 by the American Society for Microbiology. All rights reserved.