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Infection and Immunity, May 1999, p. 2619-2623, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Localization of HArep-Containing Genes on the Chromosome of Porphyromonas gingivalis W83

Janina P. Lewis, and Francis L. Macrina*

Institute of Oral and Craniofacial Molecular Biology, Virginia Commonwealth University, Richmond, Virginia 23298-0566

Received 5 October 1998/Returned for modification 24 November 1998/Accepted 4 February 1999

We have mapped a group of virulence genes of Porphyromonas gingivalis to a single large fragment of the genome. These genes (rgpA, kgp, and hagA) all contain a consensus repeat sequence (HArep). rgpA and kgp encode cysteine proteases with Arg-X and Lys-X specificity, respectively, and hagA encodes a hemagglutinin. Genomic DNA fragments separated by pulse-field gel electrophoresis were blotted and probed in order to localize the genes to a 0.25-Mb NheI fragment of the P. gingivalis W83 genome. Further hybridization analyses with single- and double-restriction digestion allowed us to generate a physical map of the fragment and determine the precise locations of the protease and hemagglutinin genes. In addition, we found an insertion-like sequence, IS195, near the ends of the 0.25-Mb NheI fragment. A similarly sized fragment carrying HArep sequences was also demonstrated in the P. gingivalis W12 and W50 genomes.

* Corresponding author. Mailing address: Institute of Oral and Craniofacial Molecular Biology, Virginia Commonwealth University, Richmond, VA 23298-0566. Phone: (804) 828-0149. Fax: (804) 828-0150. E-mail: macrina{at}vcu.edu.

Infection and Immunity, May 1999, p. 2619-2623, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Curtis, M.A., Aduse-Opoku, J., Rangarajan, M. (2001). Cysteine Proteases of Porphyromonas Gingivalis. CROBM 12: 192-216 [Abstract] [Full Text]  
  • Aduse-Opoku, J., Davies, N. N., Gallagher, A., Hashim, A., Evans, H. E. A., Rangarajan, M., Slaney, J. M., Curtis, M. A. (2000). Generation of Lys-gingipain protease activity in Porphyromonas gingivalis W50 is independent of Arg-gingipain protease activities. Microbiology 146: 1933-1940 [Abstract] [Full Text]  


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