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Infection and Immunity, May 1999, p. 2627-2632, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Characterization of the Importance of Polysaccharide Intercellular Adhesin/Hemagglutinin of Staphylococcus epidermidis in the Pathogenesis of Biomaterial-Based Infection in a Mouse Foreign Body Infection Model

Mark E. Rupp,1,* Joseph S. Ulphani,1 Paul D. Fey,1 Katrin Bartscht,2 and Dietrich Mack2

Department of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska 68198-5400,1 and Institute of Medical Microbiology and Immunology, University of Hamburg, Hamburg, Germany2

Received 10 November 1998/Returned for modification 8 December 1998/Accepted 11 February 1999

The production of biofilm is thought to be crucial in the pathogenesis of prosthetic-device infections caused by Staphylococcus epidermidis. An experimental animal model was used to assess the importance of biofilm production, which is mediated by polysaccharide intercellular adhesin/hemagglutinin (PIA/HA), in the pathogenesis of a biomaterial-based infection. Mice were inoculated along the length of a subcutaneously implanted intravenous catheter with either wild-type S. epidermidis 1457 or its isogenic PIA/HA-negative mutant. The wild-type strain was significantly more likely to cause a subcutaneous abscess than the mutant strain (P < 0.01) and was significantly less likely to be eradicated from the inoculation site by host defense (P < 0.05). In addition, the wild-type strain was found to adhere to the implanted catheters more abundantly than the PIA/HA-negative mutant (P < 0.05). The reliability of the adherence assay was assessed by scanning electron microscopy. To exclude contamination or spontaneous infection, bacterial strains recovered from the experimental animals were compared to inoculation strains by analysis of restriction fragment length polymorphism patterns by pulsed-field gel electrophoresis. In vitro binding of the wild-type strain and its isogenic mutant to a fibronectin-coated surface was similar. These results confirm the importance of biofilm production, mediated by PIA/HA, in the pathogenesis of S. epidermidis experimental foreign body infection.

* Corresponding author. Mailing address: Department of Internal Medicine, 985400 Nebraska Medical Center, Omaha, NE 68198-5400. Phone: (402) 559-8650. Fax: (402) 559-5581. E-mail: merupp{at}unmc.edu.

Infection and Immunity, May 1999, p. 2627-2632, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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