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Infection and Immunity, May 1999, p. 2649-2652, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Binding of Haemophilus ducreyi to Extracellular Matrix Proteins

Margaret E. Bauer,1,* and Stanley M. Spinola1,2,3

Departments of Medicine,1 Microbiology and Immunology,2 and Pathology and Laboratory Medicine,3 Indiana University School of Medicine, Indianapolis, Indiana 46202

Received 19 October 1998/Returned for modification 11 December 1998/Accepted 11 February 1999

We developed an enzyme-linked immunosorbent assay-based assay to assess Haemophilus ducreyi binding to extracellular matrix (ECM) proteins. H. ducreyi 35000HP bound to fibronectin, laminin, and type I and III collagen but not to type IV, V, or VI collagen or elastin. Isogenic strains with mutations in ftpA or losB bound as well as the parent, suggesting that neither pili nor full-length lipooligosaccharide is required for H. ducreyi to bind to ECM proteins.

* Corresponding author. Mailing address: Indiana University School of Medicine, Emerson Hall 435, 545 Barnhill Dr., Indianapolis, IN 46202. Phone: (317) 274-8143. Fax: (317) 274-1587. E-mail: mebauer{at}iupui.edu.

Infection and Immunity, May 1999, p. 2649-2652, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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