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Infection and Immunity, June 1999, p. 2692-2699, Vol. 67, No. 6
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Phylogenetic Analysis of Enteroaggregative and Diffusely Adherent Escherichia coli

John R. Czeczulin,1 Thomas S. Whittam,2 Ian R. Henderson,1 Fernando Navarro-Garcia,1,3 and James P. Nataro1,*

Departments of Pediatrics and Microbiology and Immunology, Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland 212011; Department of Biology, Institute of Molecular Evolutionary Genetics, The Pennsylvania State University, University Park, Pennsylvania 168022; and Department of Public Health, Faculty of Medicine, UNAM, 04510 Mexico DF, Mexico3

Received 7 January 1999/Returned for modification 3 March 1999/Accepted 16 March 1999

The phylogenetics of the various pathotypes of diarrheagenic Escherichia coli are not completely understood. In this study, we identified several plasmid and chromosomal genes in the pathogenic enteroaggregative E. coli (EAEC) prototype strain 042 and determined the prevalence of these loci among EAEC and diffusely adherent E. coli strains. The distribution of these genes is analyzed within an evolutionary framework provided by the characterization of allelic variation in housekeeping genes via multilocus enzyme electrophoresis. Our data reveal that EAEC strains are heterogeneous with respect to chromosomal and plasmid-borne genes but that the majority harbor a member of a conserved family of virulence plasmids. Comparison of plasmid and chromosomal relatedness of strains suggests clonality of chromosomal markers and a limited transfer model of plasmid distribution.

* Corresponding author. Mailing address: Center for Vaccine Development, 685 W. Baltimore St., Baltimore, MD 21201. Phone: (410) 706-8442. Fax: (410) 706-6205. E-mail: jnataro{at}umaryland.edu.

Infection and Immunity, June 1999, p. 2692-2699, Vol. 67, No. 6
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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