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Infection and Immunity, June 1999, p. 2969-2974, Vol. 67, No. 6
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Differential Induction of Colitis and Gastritis in HLA-B27 Transgenic Rats Selectively Colonized with Bacteroides vulgatus or Escherichia coli

Heiko C. Rath,1,dagger Kenneth H. Wilson,2 and R. Balfour Sartor1,*

Center of Gastrointestinal Biology and Disease, University of North Carolina, Chapel Hill, North Carolina 27599,1 and Department of Infectious Diseases, VA Medical Center, Duke University, Durham, North Carolina 277052

Received 29 October 1998/Returned for modification 15 February 1999/Accepted 16 March 1999

Resident bacteria play an important role in initiating and perpetuating gastrointestinal inflammation. We previously demonstrated that six commensal bacteria including Bacteroides vulgatus caused more aggressive colitis and gastritis in HLA-B27 transgenic rats than did the other five bacteria without B. vulgatus. This study compared the degree of gastrointestinal inflammation in gnotobiotic HLA-B27 transgenic rats monoassociated with either B. vulgatus or Escherichia coli. Gnotobiotic transgenic rats raised in Trexler isolators were selectively colonized with either B. vulgatus or E. coli. Control rats were either germfree or colonized with six common commensal bacteria (Streptococcus faecium, E. coli, Streptococcus avium, Eubacterium contortum, Peptostreptococcus productus, and B. vulgatus [DESEP-B]). After 1 month, all the rats were killed and tissues were prepared for histologic and biochemical evaluation. Colitis induced by B. vulgatus monoassociation was almost equal to that in DESEP-B-colonized rats and was significantly more severe than E. coli-induced colitis, which was absent by histological testing and mild by colonic myeloperoxidase and interleukin-1beta concentration determinations. However, gastritis was detectable only in DESEP-B-associated rats. These studies suggest that not all resident bacteria have equal proinflammatory capabilities, since B. vulgatus alone is more active than E. coli alone in inducing colitis, and that colitis and gastritis result from different luminal bacterial stimuli.

* Corresponding author. Mailing address: Division of Digestive Diseases, CB 7080, Room 030, Glaxo Bldg., University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7080. Phone: (919) 966-0149. Fax: (919) 966-7468. E-mail: rbs{at}med.unc.edu.

dagger Present address: Klinik und Poliklinik für Innere Medizin I, Universität Regensburg, Regensburg, Germany.

Infection and Immunity, June 1999, p. 2969-2974, Vol. 67, No. 6
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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