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Infection and Immunity, June 1999, p. 3014-3018, Vol. 67, No. 6
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Interleukin 5 (IL-5) Is Not Required for Expression of a Th2 Response or Host Resistance Mechanisms during Murine Schistosomiasis Mansoni but Does Play a Role in Development of IL-4-Producing Non-T, Non-B Cells

Laura Rosa Brunet,1,dagger Elizabeth A. Sabin,1,Dagger Allen W. Cheever,2 Manfred A. Kopf,3 and Edward J. Pearce1,*

Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York 148531; Biomedical Research Institute, Rockville, Maryland 208522; and Basel Institute for Immunology, Basel, Switzerland3

Received 12 November 1998/Returned for modification 17 December 1998/Accepted 26 March 1999

During schistosomiasis, interleukin-5 (IL-5)-dependent eosinophil responses have been implicated in immunopathology, resistance to superinfection, synergistic interactions with chemotherapeutic agents, and the inductive phase of the egg-induced Th2 response. We examined these issues in IL-5-deficient (IL-5-/-) mice. IL-5-/- and wild-type (WT) mice were indistinguishable in terms of susceptibility to primary infections and the ability to resist secondary infections. Moreover, hepatic pathology was similar in both strains apart from a relative lack of eosinophils and, during chronic infection, a significantly larger mast cell component in the granulomas of IL-5-/- mice. Splenocyte cytokine production in response to soluble egg antigen (SEA) or anti-CD3 revealed no significant differences except for heightened tumor necrosis factor alpha production by cells from chronically infected IL-5-/- mice compared to WT animals. In contrast, ionomycin-stimulated non-B, non-T (NBNT) cells from IL-5-/- mice produced significantly smaller IL-4 amounts than did NBNT cells from WT animals. This difference was not apparent following plate-bound anti-immunoglobulin E or SEA stimulation. The absence of IL-5 failed to affect the induction of Th2 responses in naive mice. Peritoneal exudate cells recovered from egg-injected IL-5-/- or WT mice produced equivalent levels of IL-4 following restimulation with SEA or anti-CD3.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, C5-165 VMC, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853-6401. Phone: (607) 253-3389. Fax: (607) 253-3384. E-mail: ejp2{at}cornell.edu.

dagger Present address: Department of Pathology, Cambridge University, Cambridge, United Kingdom CB2 1QP.

Dagger Present address: JAVMA, Schaumburg, IL 60173-4360.

Infection and Immunity, June 1999, p. 3014-3018, Vol. 67, No. 6
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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