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Infection and Immunity, June 1999, p. 3141-3145, Vol. 67, No. 6
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

FbpC Is Not Essential for Iron Acquisition in Neisseria gonorrhoeae

Shite Sebastian, and Caroline Attardo Genco*

The Maxwell Finland Laboratory for Infectious Diseases, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118

Received 8 December 1998/Returned for modification 13 January 1999/Accepted 12 March 1999

The fbpABC locus of Neisseria gonorrhoeae has been proposed to encode a periplasmic protein-dependent iron transport system. Although the function of the gonococcal FbpA protein has been well characterized and its role as a periplasmic binding protein is well defined, little is known about the function of the FbpB and FbpC proteins. To define the function of the gonococcal FbpC protein, an N. gonorrhoeae F62 fbpC mutant was constructed by insertional inactivation with the kanamycin gene. The N. gonorrhoeae F62 fbpC mutant was observed to grow with heme, transferrin, or ferric nitrate as the sole exogenous iron source, indicating that the gonococcal FbpC protein is not absolutely required for growth with these iron sources. In previous studies we were unable to detect fbpB- or fbpC-specific transcripts by Northern analysis. Reverse transcription-PCR analysis with RNA obtained from N. gonorrhoeae F62 grown under iron-replete and -depleted conditions detected fbpA and fbpAB transcripts but failed to detect fbpC or fbpBC transcripts. These results indicate that FbpC does not play a pivotal role in iron transport in N. gonorrhoeae and suggest that additional ABC transport systems are functional in the gonococcus for the acquisition of iron.

* Corresponding author. Mailing address: The Maxwell Finland Laboratory for Infectious Diseases, Boston University School of Medicine, Department of Medicine, Boston, MA 02118. Phone: (617) 414-5282. Fax: (617) 414-5280. E-mail: caroline.genco{at}bmc.org.

Infection and Immunity, June 1999, p. 3141-3145, Vol. 67, No. 6
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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