Previous Article
Infection and Immunity, June 1999, p. 3175-3179, Vol. 67, No. 6
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Lymphotoxin Inhibits Chlamydia
pneumoniae Growth in HEp-2 Cells
Hiroshi
Matsushima,1,2
Mutsunori
Shirai,1
Kazunobu
Ouchi,3
Kenji
Yamashita,4
Tetsu
Kakutani,4
Susumu
Furukawa,2 and
Teruko
Nakazawa1,*
Department of
Microbiology1 and Department of
Pediatrics,2 Yamaguchi University School of
Medicine, Ube, Yamaguchi 755-8505, Saiseikai Shimonoseki
General Hospital, Shimonoseki 751-0823,3 and
Kanegafuchi Chemical Industry Co., Takasago, Hyogo
676-8688,4 Japan
Received 3 November 1998/Returned for modification 13 January
1999/Accepted 12 March 1999
Cytokines such as gamma interferon and tumor necrosis factor alpha
(TNF-
) inhibit the intracellular replication of Chlamydia pneumoniae or Chlamydia trachomatis. In this study,
we found that another cytokine, lymphotoxin (TNF-
), restricts the
growth of C. pneumoniae in HEp-2 cells. When lymphotoxin
(10 U/ml) was added during incubation from 8 to 16 h
postinoculation, inclusion body formation was severely reduced. In
addition, we observed activation of nitric oxide production and the
nuclear transition of NF-
B in HEp-2 cells in response to
lymphotoxin. These results suggest that inhibition of chlamydial growth
by lymphotoxin is mediated, at least in part, by nuclear transition of
NF-
B, resulting in induction of nitric oxide synthase to produce
nitric oxide, a potent bacteristatic agent. This is the first report on
antichlamydial activity of lymphotoxin through induction of nitric oxide.
*
Corresponding author. Mailing address: Department of
Microbiology, Yamaguchi University School of Medicine, Ube, Yamaguchi 755-8505, Japan. Phone: 81-836-22-2226. Fax: 81-836-22-2415. E-mail: nakazawa{at}po.cc.yamaguchi-u.ac.jp.
Infection and Immunity, June 1999, p. 3175-3179, Vol. 67, No. 6
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Vanover, J., Sun, J., Deka, S., Kintner, J., Duffourc, M. M., Schoborg, R. V.
(2008). Herpes simplex virus co-infection-induced Chlamydia trachomatis persistence is not mediated by any known persistence inducer or anti-chlamydial pathway. Microbiology
154: 971-978
[Abstract]
[Full Text]
-
Sakai, K., Suzuki, H., Oda, H., Akaike, T., Azuma, Y., Murakami, T., Sugi, K., Ito, T., Ichinose, H., Koyasu, S., Shirai, M.
(2006). Phosphoinositide 3-Kinase in Nitric Oxide Synthesis in Macrophage: CRITICAL DIMERIZATION OF INDUCIBLE NITRIC-OXIDE SYNTHASE. J. Biol. Chem.
281: 17736-17742
[Abstract]
[Full Text]
-
Azuma, Y., Hirakawa, H., Yamashita, A., Cai, Y., Rahman, M. A., Suzuki, H., Mitaku, S., Toh, H., Goto, S., Murakami, T., Sugi, K., Hayashi, H., Fukushi, H., Hattori, M., Kuhara, S., Shirai, M.
(2006). Genome Sequence of the Cat Pathogen, Chlamydophila felis. DNA Res
13: 15-23
[Abstract]
[Full Text]
-
Rahman, Mohd. A., Azuma, Y., Fukunaga, H., Murakami, T., Sugi, K., Fukushi, H., Miura, K., Suzuki, H., Shirai, M.
(2005). Serotonin and melatonin, neurohormones for homeostasis, as novel inhibitors of infections by the intracellular parasite chlamydia. J Antimicrob Chemother
56: 861-868
[Abstract]
[Full Text]
-
Yoneda, H., Miura, K., Matsushima, H., Sugi, K., Murakami, T., Ouchi, K., Yamashita, K., Itoh, H., Nakazawa, T., Suzuki, M., Shirai, M.
(2003). Aspirin inhibits Chlamydia pneumoniae-induced NF-{kappa}B activation, cyclo-oxygenase-2 expression and prostaglandin E2 synthesis and attenuates chlamydial growth. J Med Microbiol
52: 409-415
[Abstract]
[Full Text]
-
Yang, J., Hooper, W. C., Phillips, D. J., Tondella, M. L., Talkington, D. F.
(2003). Induction of Proinflammatory Cytokines in Human Lung Epithelial Cells during Chlamydia pneumoniae Infection. Infect. Immun.
71: 614-620
[Abstract]
[Full Text]
-
Tiran, A., Gruber, H.-J., Graier, W. F., Wagner, A. H., van Leeuwen, E. B.M., Tiran, B.
(2002). Aspirin Inhibits Chlamydia pneumoniae-Induced Nuclear Factor-{kappa}B Activation, Cytokine Expression, and Bacterial Development in Human Endothelial Cells. Arterioscler. Thromb. Vasc. Bio.
22: 1075-1080
[Abstract]
[Full Text]
-
Roach, D. R., Bean, A. G. D., Demangel, C., France, M. P., Briscoe, H., Britton, W. J.
(2002). TNF Regulates Chemokine Induction Essential for Cell Recruitment, Granuloma Formation, and Clearance of Mycobacterial Infection. J. Immunol.
168: 4620-4627
[Abstract]
[Full Text]
-
Prebeck, S., Kirschning, C., Durr, S., da Costa, C., Donath, B., Brand, K., Redecke, V., Wagner, H., Miethke, T.
(2001). Predominant Role of Toll-Like Receptor 2 Versus 4 in Chlamydia pneumoniae-Induced Activation of Dendritic Cells. J. Immunol.
167: 3316-3323
[Abstract]
[Full Text]
-
Roach, D. R., Briscoe, H., Saunders, B., France, M. P., Riminton, S., Britton, W. J.
(2001). Secreted Lymphotoxin-{alpha} Is Essential for the Control of an Intracellular Bacterial Infection. JEM
193: 239-246
[Abstract]
[Full Text]
-
Miura, K., Inouye, S., Sakai, K., Takaoka, H., Kishi, F., Tabuchi, M., Tanaka, T., Matsumoto, H., Shirai, M., Nakazawa, T., Nakazawa, A.
(2001). Cloning and Characterization of Adenylate Kinase from Chlamydia pneumoniae. J. Biol. Chem.
276: 13490-13498
[Abstract]
[Full Text]