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Infection and Immunity, July 1999, p. 3587-3592, Vol. 67, No. 7
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Protective Immunity of Microsphere-Based Mucosal Vaccines against Lethal Intranasal Challenge with Streptococcus pneumoniae

Seung-Yong Seong,dagger Nam-Hyuk Cho,dagger Ick Chan Kwon, and Seo Young Jeong*

Biomedical Research Center, Korea Institute of Science and Technology, Seoul 136-791, Korea

Received 24 August 1998/Returned for modification 26 February 1999/Accepted 9 April 1999

Mucosal vaccination of capsular polysaccharide (PS) of Streptococcus pneumoniae and subsequent creation of the first line of immunological defense in mucosa were examined. Mucosal as well as systemic antibody responses to PS were evoked by peroral or intranasal immunization of BALB/c mice with PS-cholera toxin B subunit (CTB) conjugates entrapped in the alginate microspheres (AM). The bacterial colonization at the lung mucosa was most profoundly inhibited (<95%) by intranasal immunization with the naked conjugate (PS-CTB). The mice vaccinated orally with encapsulated conjugate [AM(PS-CTB)] showed significant reduction on the level of pneumococcal bacteremia (<99%). Eighty percent of the mice perorally immunized with AM (PS-CTB) were protected from lethal intranasal challenge with S. pneumoniae, whereas more than 60% of the mice in the other control groups died of infection. Our novel approach may prove to be important in the development of a mucosal vaccine that will provide protection of mucosal surfaces of host.

* Corresponding author. Mailing address: Biomedical Research Center, Korea Institute of Science and Technology, Seoul 136-791, Korea. Phone: 82-2-958-5911. Fax: 82-2-958-5909. E-mail: syjeong{at}kist.re.kr.

dagger Present address: Department of Microbiology, Seoul National University College of Medicine, Seoul 110-799, Korea.

Infection and Immunity, July 1999, p. 3587-3592, Vol. 67, No. 7
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Seo, J.-Y., Seong, S. Y., Ahn, B.-Y., Kwon, I. C., Chung, H., Jeong, S. Y. (2002). Cross-Protective Immunity of Mice Induced by Oral Immunization with Pneumococcal Surface Adhesin A Encapsulated in Microspheres. Infect. Immun. 70: 1143-1149 [Abstract] [Full Text]  
  • Jakobsen, H., Bjarnarson, S., Del Giudice, G., Moreau, M., Siegrist, C.-A., Jonsdottir, I. (2002). Intranasal Immunization with Pneumococcal Conjugate Vaccines with LT-K63, a Nontoxic Mutant of Heat-Labile Enterotoxin, as Adjuvant Rapidly Induces Protective Immunity against Lethal Pneumococcal Infections in Neonatal Mice. Infect. Immun. 70: 1443-1452 [Abstract] [Full Text]  
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