This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wyant, T. L.
Right arrow Articles by Sztein, M. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wyant, T. L.
Right arrow Articles by Sztein, M. B.

 Previous Article  |  Next Article 

Infection and Immunity, July 1999, p. 3619-3624, Vol. 67, No. 7
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Salmonella typhi Flagella Are Potent Inducers of Proinflammatory Cytokine Secretion by Human Monocytes

Timothy L. Wyant,dagger Michael K. Tanner, and Marcelo B. Sztein*

Center for Vaccine Development, Departments of Pediatrics and Medicine, University of Maryland, Baltimore, Maryland 21201

Received 25 January 1999/Returned for modification 19 March 1999/Accepted 21 April 1999

The cytokine production patterns of human peripheral blood mononuclear cells (PBMC) in response to Salmonella typhi flagella (STF) were examined in culture supernatants of PBMC stimulated with STF. Consistent with previous findings in volunteers vaccinated with aroC aroD deletion mutants of S. typhi, PBMC from volunteers immunized with the licensed live Ty21a S. typhi vaccine secreted gamma interferon following exposure to STF. Stimulation with STF induced rapid de novo synthesis of tumor necrosis factor alpha (TNF-alpha ) and interleukin-1beta (IL-1beta ), followed by IL-6 and IL-10. Trypsin treatment of STF abrogated their effects, while polymyxin B had no effect. Intracellular cytokine measurements of STF-stimulated PBMC revealed the existence of monocyte subpopulations that produce only TNF-alpha , IL-1beta or both cytokines. Moreover, STF markedly decreased the percentage of CD14+ cells. These data demonstrate that STF are powerful monocyte activators which may have important implications for vaccine development and for understanding the pathogenesis of S. typhi infection.


* Corresponding author. Mailing address: Center for Vaccine Development, Departments of Pediatrics and Medicine, University of Maryland, 685 West Baltimore St., Rm. 480, Baltimore, MD 21201. Phone: (410) 706-2345. Fax: (410) 706-6205. E-mail: msztein{at}UMPPA1.ab.umd.edu.

dagger Present address: SurroMed,Inc., Palo Alto, CA 94303.


Infection and Immunity, July 1999, p. 3619-3624, Vol. 67, No. 7
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Salazar-Gonzalez, R.-M., Srinivasan, A., Griffin, A., Muralimohan, G., Ertelt, J. M., Ravindran, R., Vella, A. T., McSorley, S. J. (2007). Salmonella Flagellin Induces Bystander Activation of Splenic Dendritic Cells and Hinders Bacterial Replication In Vivo. J. Immunol. 179: 6169-6175 [Abstract] [Full Text]  
  • McNally, A., La Ragione, R. M., Best, A., Manning, G., Newell, D. G. (2007). An aflagellate mutant Yersinia enterocolitica biotype 1A strain displays altered invasion of epithelial cells, persistence in macrophages, and cytokine secretion profiles in vitro. Microbiology 153: 1339-1349 [Abstract] [Full Text]  
  • Steiner, T. S. (2007). How Flagellin and Toll-Like Receptor 5 Contribute to Enteric Infection. Infect. Immun. 75: 545-552 [Full Text]  
  • Raffatellu, M., Chessa, D., Wilson, R. P., Tukel, C., Akcelik, M., Baumler, A. J. (2006). Capsule-Mediated Immune Evasion: a New Hypothesis Explaining Aspects of Typhoid Fever Pathogenesis. Infect. Immun. 74: 19-27 [Full Text]  
  • Thamm, D. H., Kurzman, I. D., King, I., Li, Z., Sznol, M., Dubielzig, R. R., Vail, D. M., MacEwen, E. G. (2005). Systemic Administration of an Attenuated, Tumor-Targeting Salmonella typhimurium to Dogs with Spontaneous Neoplasia: Phase I Evaluation. Clin. Cancer Res. 11: 4827-4834 [Abstract] [Full Text]  
  • Raffatellu, M., Chessa, D., Wilson, R. P., Dusold, R., Rubino, S., Baumler, A. J. (2005). The Vi Capsular Antigen of Salmonella enterica Serotype Typhi Reduces Toll-Like Receptor-Dependent Interleukin-8 Expression in the Intestinal Mucosa. Infect. Immun. 73: 3367-3374 [Abstract] [Full Text]  
  • Dons, L., Eriksson, E., Jin, Y., Rottenberg, M. E., Kristensson, K., Larsen, C. N., Bresciani, J., Olsen, J. E. (2004). Role of Flagellin and the Two-Component CheA/CheY System of Listeria monocytogenes in Host Cell Invasion and Virulence. Infect. Immun. 72: 3237-3244 [Abstract] [Full Text]  
  • Adamo, R., Sokol, S., Soong, G., Gomez, M. I., Prince, A. (2004). Pseudomonas aeruginosa Flagella Activate Airway Epithelial Cells through asialoGM1 and Toll-Like Receptor 2 as well as Toll-Like Receptor 5. Am. J. Respir. Cell Mol. Bio. 30: 627-634 [Abstract] [Full Text]  
  • Peters, K., Unger, R. E., Brunner, J., Kirkpatrick, C.J. (2003). Molecular basis of endothelial dysfunction in sepsis. Cardiovasc Res 60: 49-57 [Abstract] [Full Text]  
  • Zeng, H., Carlson, A. Q., Guo, Y., Yu, Y., Collier-Hyams, L. S., Madara, J. L., Gewirtz, A. T., Neish, A. S. (2003). Flagellin Is the Major Proinflammatory Determinant of Enteropathogenic Salmonella. J. Immunol. 171: 3668-3674 [Abstract] [Full Text]  
  • Salerno-Goncalves, R., Wyant, T. L., Pasetti, M. F., Fernandez-Vina, M., Tacket, C. O., Levine, M. M., Sztein, M. B. (2003). Concomitant Induction of CD4+ and CD8+ T Cell Responses in Volunteers Immunized with Salmonella enterica Serovar Typhi Strain CVD 908-htrA. J. Immunol. 170: 2734-2741 [Abstract] [Full Text]  
  • Donnelly, M. A., Steiner, T. S. (2002). Two Nonadjacent Regions in Enteroaggregative Escherichia coli Flagellin Are Required for Activation of Toll-like Receptor 5. J. Biol. Chem. 277: 40456-40461 [Abstract] [Full Text]  
  • McSorley, S. J., Ehst, B. D., Yu, Y., Gewirtz, A. T. (2002). Bacterial Flagellin Is an Effective Adjuvant for CD4+ T Cells In Vivo. J. Immunol. 169: 3914-3919 [Abstract] [Full Text]  
  • Salerno-Goncalves, R., Pasetti, M. F., Sztein, M. B. (2002). Characterization of CD8+ Effector T Cell Responses in Volunteers Immunized with Salmonella enterica Serovar Typhi Strain Ty21a Typhoid Vaccine. J. Immunol. 169: 2196-2203 [Abstract] [Full Text]  
  • Pasetti, M. F., Salerno-Goncalves, R., Sztein, M. B. (2002). Salmonella enterica Serovar Typhi Live Vector Vaccines Delivered Intranasally Elicit Regional and Systemic Specific CD8+ Major Histocompatibility Class I-Restricted Cytotoxic T Lymphocytes. Infect. Immun. 70: 4009-4018 [Abstract] [Full Text]  
  • Strindelius, L., Wikingsson, L. D., Sjoholm, I. (2002). Extracellular Antigens from Salmonella enteritidis Induce Effective Immune Response in Mice after Oral Vaccination. Infect. Immun. 70: 1434-1442 [Abstract] [Full Text]  
  • Boldrick, J. C., Alizadeh, A. A., Diehn, M., Dudoit, S., Liu, C. L., Belcher, C. E., Botstein, D., Staudt, L. M., Brown, P. O., Relman, D. A. (2002). Stereotyped and specific gene expression programs in human innate immune responses to bacteria. Proc. Natl. Acad. Sci. USA 99: 972-977 [Abstract] [Full Text]  
  • Eaves-Pyles, T. D., Wong, H. R., Odoms, K., Pyles, R. B. (2001). Salmonella Flagellin-Dependent Proinflammatory Responses Are Localized to the Conserved Amino and Carboxyl Regions of the Protein. J. Immunol. 167: 7009-7016 [Abstract] [Full Text]  
  • Schmitt, C. K., Ikeda, J. S., Darnell, S. C., Watson, P. R., Bispham, J., Wallis, T. S., Weinstein, D. L., Metcalf, E. S., O'Brien, A. D. (2001). Absence of All Components of the Flagellar Export and Synthesis Machinery Differentially Alters Virulence of Salmonella enterica Serovar Typhimurium in Models of Typhoid Fever, Survival in Macrophages, Tissue Culture Invasiveness, and Calf Enterocolitis. Infect. Immun. 69: 5619-5625 [Abstract] [Full Text]  
  • Wang, J. Y., Pasetti, M. F., Noriega, F. R., Anderson, R. J., Wasserman, S. S., Galen, J. E., Sztein, M. B., Levine, M. M. (2001). Construction, Genotypic and Phenotypic Characterization, and Immunogenicity of Attenuated {Delta}guaBA Salmonella enterica Serovar Typhi Strain CVD 915. Infect. Immun. 69: 4734-4741 [Abstract] [Full Text]  
  • Moors, M. A., Li, L., Mizel, S. B. (2001). Activation of Interleukin-1 Receptor-Associated Kinase by Gram-Negative Flagellin. Infect. Immun. 69: 4424-4429 [Abstract] [Full Text]  
  • Kotloff, K. L., Sztein, M. B., Wasserman, S. S., Losonsky, G. A., DiLorenzo, S. C., Walker, R. I. (2001). Safety and Immunogenicity of Oral Inactivated Whole-Cell Helicobacter pylori Vaccine with Adjuvant among Volunteers with or without Subclinical Infection. Infect. Immun. 69: 3581-3590 [Abstract] [Full Text]  
  • McDermott, P. F., Ciacci-Woolwine, F., Snipes, J. A., Mizel, S. B. (2000). High-Affinity Interaction between Gram-Negative Flagellin and a Cell Surface Polypeptide Results in Human Monocyte Activation. Infect. Immun. 68: 5525-5529 [Abstract] [Full Text]  
  • Ogushi, K.-i., Wada, A., Niidome, T., Mori, N., Oishi, K., Nagatake, T., Takahashi, A., Asakura, H., Makino, S.-i., Hojo, H., Nakahara, Y., Ohsaki, M., Hatakeyama, T., Aoyagi, H., Kurazono, H., Moss, J., Hirayama, T. (2001). Salmonella enteritidis FliC (Flagella Filament Protein) Induces Human beta -Defensin-2 mRNA Production by Caco-2 Cells. J. Biol. Chem. 276: 30521-30526 [Abstract] [Full Text]