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Infection and Immunity, July 1999, p. 3653-3656, Vol. 67, No. 7
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Development of a System for Expressing Heterologous Genes in the Oral Spirochete Treponema denticola and Its Use in Expression of the Treponema pallidum flaA Gene

Bo Chi, Sarita Chauhan,dagger and Howard Kuramitsu*

Department of Oral Biology, State University of New York at Buffalo, Buffalo, New York 14214

Received 16 February 1999/Returned for modification 8 April 1999/Accepted 19 April 1999

The present communication describes the construction of a new Escherichia coli-Treponema denticola shuttle vector based on the naturally occurring spirochete plasmid pTS1 and the expression of the heterologous T. pallidum flaA gene from the plasmid in T. denticola. This new shuttle vector system should prove useful in characterizing virulence factors from unculturable pathogenic spirochetes.

* Corresponding author. Mailing address: Department of Oral Biology, State University of New York at Buffalo, 3435 Main St., Buffalo, NY 14214-3092. Phone: (716) 829-2068. Fax: (716) 829-3942. E-mail: kuramits{at}acsu.buffalo.edu.

dagger Present address: Dupont Central Research and Development, Wilmington, DE 19880.

Infection and Immunity, July 1999, p. 3653-3656, Vol. 67, No. 7
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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