This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Island, M. D.
Right arrow Articles by Warren, J. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Island, M. D.
Right arrow Articles by Warren, J. W.

 Previous Article  |  Next Article 

Infection and Immunity, July 1999, p. 3657-3661, Vol. 67, No. 7
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Effect of Escherichia coli Cytotoxic Necrotizing Factor 1 on Repair of Human Bladder Cell Monolayers In Vitro

Michael D. Island,* Xaioling Cui, and John W. Warren

Division of Infectious Diseases, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland

Received 17 December 1998/Returned for modification 29 January 1999/Accepted 31 March 1999

We hypothesized that Escherichia coli cytotoxic necrotizing factor 1 (CNF1) might impair migration or proliferation of bladder cells and could potentially interfere with repair of the bladder epithelium. Using experimentally wounded human T24 bladder epithelial cell monolayers as an in vitro model, we found that both the number of T24 cells and the maximum distance they migrated into wounded regions was significantly decreased by bacterial extracts containing E. coli CNF1.

* Corresponding author. Mailing address: Division of Infectious Diseases, Department of Medicine, University of Maryland School of Medicine, 10 S. Pine St., Baltimore, MD 21201-1192. Phone: (410) 706-7560. Fax: (410) 706-8700. E-mail: misland{at}clark.net.

Infection and Immunity, July 1999, p. 3657-3661, Vol. 67, No. 7
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Hopkins, A. M., Walsh, S. V., Verkade, P., Boquet, P., Nusrat, A. (2003). Constitutive activation of Rho proteins by CNF-1 influences tight junction structure and epithelial barrier function. J. Cell Sci. 116: 725-742 [Abstract] [Full Text]  
  • Rippere-Lampe, K. E., Lang, M., Ceri, H., Olson, M., Lockman, H. A., O'Brien, A. D. (2001). Cytotoxic Necrotizing Factor Type 1-Positive Escherichia coli Causes Increased Inflammation and Tissue Damage to the Prostate in a Rat Prostatitis Model. Infect. Immun. 69: 6515-6519 [Abstract] [Full Text]  
  • Rippere-Lampe, K. E., O'Brien, A. D., Conran, R., Lockman, H. A. (2001). Mutation of the Gene Encoding Cytotoxic Necrotizing Factor Type 1 (cnf1) Attenuates the Virulence of Uropathogenic Escherichia coli. Infect. Immun. 69: 3954-3964 [Abstract] [Full Text]  
  • Mills, M., Meysick, K. C., O'Brien, A. D. (2000). Cytotoxic Necrotizing Factor Type 1 of Uropathogenic Escherichia coli Kills Cultured Human Uroepithelial 5637 Cells by an Apoptotic Mechanism. Infect. Immun. 68: 5869-5880 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»