Legionella pneumophila Contains a Type II General Secretion Pathway Required for Growth in Amoebae as Well as for Secretion of the Msp Protease

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Infection and Immunity, July 1999, p. 3662-3666, Vol. 67, No. 7
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Legionella pneumophila Contains a Type II General Secretion Pathway Required for Growth in Amoebae as Well as for Secretion of the Msp Protease

Laura M. Hales, and Howard A. Shuman^*

Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032

Received 11 December 1998/Returned for modification 18 January 1999/Accepted 19 April 1999

We report the identification of a set of Legionella pneumophila genes that encode products with homology to proteins of thetype II general secretion pathway of gram-negative bacteria. Astrain containing a deletion-substitution mutation of two of thesegenes was unable to secrete the Msp protease. This strain wasunable to multiply within the free-living amoeba Acanthamoebacastellanii yet was able to kill HL-60-derived macrophages. BecauseMsp is not required for growth in amoebae, other proteins whichare important for growth in amoebae are likely secreted by thispathway.

^* Corresponding author. Mailing address: Department of Microbiology, College of Physicians and Surgeons, Columbia University,701 West 168th St., New York, NY 10032. Phone: (212) 305-6913.Fax: (212) 305-1468. E-mail: shuman{at}cuccfa.ccc.columbia.edu.

Infection and Immunity, July 1999, p. 3662-3666, Vol. 67, No. 7
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

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