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Infection and Immunity, July 1999, p. 3662-3666, Vol. 67, No. 7
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Legionella pneumophila Contains a Type II General Secretion Pathway Required for Growth in Amoebae as Well as for Secretion of the Msp Protease

Laura M. Hales, and Howard A. Shuman*

Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032

Received 11 December 1998/Returned for modification 18 January 1999/Accepted 19 April 1999

We report the identification of a set of Legionella pneumophila genes that encode products with homology to proteins of the type II general secretion pathway of gram-negative bacteria. A strain containing a deletion-substitution mutation of two of these genes was unable to secrete the Msp protease. This strain was unable to multiply within the free-living amoeba Acanthamoeba castellanii yet was able to kill HL-60-derived macrophages. Because Msp is not required for growth in amoebae, other proteins which are important for growth in amoebae are likely secreted by this pathway.

* Corresponding author. Mailing address: Department of Microbiology, College of Physicians and Surgeons, Columbia University, 701 West 168th St., New York, NY 10032. Phone: (212) 305-6913. Fax: (212) 305-1468. E-mail: shuman{at}cuccfa.ccc.columbia.edu.

Infection and Immunity, July 1999, p. 3662-3666, Vol. 67, No. 7
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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