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Infection and Immunity, July 1999, p. 3667-3669, Vol. 67, No. 7
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Isolation and Characterization of a sigB Deletion Mutant of Staphylococcus aureus

R. O. Nicholas,* Tong Li, D. McDevitt, Andrea Marra,dagger S. Sucoloski, P. L. Demarsh, and D. R. Gentry

SmithKline Beecham Pharmaceuticals, Collegeville, Pennsylvania

Received 13 January 1999/Returned for modification 15 February 1999/Accepted 13 April 1999

The sigB gene of Staphylococcus aureus, coding for the alternate sigma factor B, has been deleted by allelic replacement mutagenesis. The mutant grew as well as the parent in vitro, although it was deficient in clumping factor, coagulase, and pigment. In two murine and one rat infection model the mutant showed no reduction in virulence.

* Corresponding author. Mailing address: Anti-infectives Research, SmithKline Beecham Pharmaceuticals, 1250 S. Collegeville Rd., P.O. Box 5089, Collegeville, PA 19426-0989. Phone: (610) 917-7378. Fax: (610) 917-7901. E-mail: richard_o_nicholas{at}sbphrd.com.

dagger Present address: Protein Design Labs, Inc., Fremont, CA 94555.

Infection and Immunity, July 1999, p. 3667-3669, Vol. 67, No. 7
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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