This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Benyacoub, J.
Right arrow Articles by Nardelli-Haefliger, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Benyacoub, J.
Right arrow Articles by Nardelli-Haefliger, D.

 Previous Article  |  Next Article 

Infection and Immunity, July 1999, p. 3674-3679, Vol. 67, No. 7
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The Nature of the Attenuation of Salmonella typhimurium Strains Expressing Human Papillomavirus Type 16 Virus-Like Particles Determines the Systemic and Mucosal Antibody Responses in Nasally Immunized Mice

Jalil Benyacoub,1 Sally Hopkins,2 Alexandra Potts,1 Sandra Kelly,3 Jean-Pierre Kraehenbuhl,2 Roy Curtiss III,3 Pierre De Grandi,1 and Denise Nardelli-Haefliger1,*

Department of Gynecology, Centre Hospitalier Universitaire Vaudois, CH-1011 Lausanne,1 and Institute of Biochemistry, University of Lausanne, and Swiss Institute for Cancer Research, CH-1066 Epalinges,2 Switzerland, and Department of Biology, Washington University, St. Louis, Missouri 631303

Received 28 December 1998/Returned for modification 9 March 1999/Accepted 26 April 1999

We have recently shown by using a recombinant Salmonella typhimurium PhoPc strain in mice the feasibility of using a Salmonella-based vaccine to prevent infection by the genital human papillomavirus type 16 (HPV16). Here, we compare the HPV16-specific antibody responses elicited by nasal immunization with recombinant S. typhimurium strains harboring attenuations that, in contrast to PhoPc, are suitable for human use. For this purpose, chi 4989 (Delta cya Delta crp) and chi 4990 [Delta cya Delta (crp-cdt)] were constructed in the ATCC 14028 genetic background, and comparison was made with the isogenic PhoPc and PhoP- strains. Although the levels of expression of HPV16 virus-like particle (VLP) were similar in all strains, only PhoPc HPV16 induced sustained specific antibody responses after nasal immunization, while all strains induced high antibody responses with a single nasal immunization when an unrelated viral hepatitis B core antigen was expressed. The level of the specific antibody responses induced did not correlate with the number of recombinant bacteria surviving in various organs 2 weeks after immunization. Our data suggest that the immunogenicity of attenuated Salmonella vaccine strains does not correlate with either the number of persisting bacteria after immunization or the levels of in vitro expression of the antigen carried. Rather, the PhoPc phenotype appears to provide the unique ability in Salmonella to induce immune responses against HPV16 VLPs.

* Corresponding author. Mailing address: Département de Gynécologie, c/o Institut de Microbiologie, Bugnon 44, 1011 Lausanne, Switzerland. Phone: 021/314 40 81. Fax: 021/314 40 95. E-mail: DNARDELL{at}hola.hospvd.ch.

Infection and Immunity, July 1999, p. 3674-3679, Vol. 67, No. 7
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Echchannaoui, H., Bianchi, M., Baud, D., Bobst, M., Stehle, J.-C., Nardelli-Haefliger, D. (2008). Intravaginal Immunization of Mice with Recombinant Salmonella enterica Serovar Typhimurium Expressing Human Papillomavirus Type 16 Antigens as a Potential Route of Vaccination against Cervical Cancer. Infect. Immun. 76: 1940-1951 [Abstract] [Full Text]  
  • Fraillery, D., Baud, D., Pang, S. Y.-Y., Schiller, J., Bobst, M., Zosso, N., Ponci, F., Nardelli-Haefliger, D. (2007). Salmonella enterica Serovar Typhi Ty21a Expressing Human Papillomavirus Type 16 L1 as a Potential Live Vaccine against Cervical Cancer and Typhoid Fever. CVI 14: 1285-1295 [Abstract] [Full Text]  
  • Hu, H., Lu, X., Tao, L., Bai, B., Zhang, Z., Chen, Y., Zheng, F., Chen, J., Chen, Z., Wang, H. (2007). Induction of Specific Immune Responses by Severe Acute Respiratory Syndrome Coronavirus Spike DNA Vaccine with or without Interleukin-2 Immunization Using Different Vaccination Routes in Mice. CVI 14: 894-901 [Abstract] [Full Text]  
  • Baud, D., Ponci, F., Bobst, M., De Grandi, P., Nardelli-Haefliger, D. (2004). Improved Efficiency of a Salmonella-Based Vaccine against Human Papillomavirus Type 16 Virus-Like Particles Achieved by Using a Codon-Optimized Version of L1. J. Virol. 78: 12901-12909 [Abstract] [Full Text]  
  • Kotton, C. N., Hohmann, E. L. (2004). Enteric Pathogens as Vaccine Vectors for Foreign Antigen Delivery. Infect. Immun. 72: 5535-5547 [Full Text]  
  • Baud, D., Benyacoub, J., Revaz, V., Kok, M., Ponci, F., Bobst, M., Curtiss, R. III, De Grandi, P., Nardelli-Haefliger, D. (2004). Immunogenicity against Human Papillomavirus Type 16 Virus-Like Particles Is Strongly Enhanced by the PhoPc Phenotype in Salmonella enterica Serovar Typhimurium. Infect. Immun. 72: 750-756 [Abstract] [Full Text]  
  • Newcombe, J., Eales-Reynolds, L.-J., Wootton, L., Gorringe, A. R., Funnell, S. G. P., Taylor, S. C., McFadden, J. J. (2004). Infection with an Avirulent phoP Mutant of Neisseria meningitidis Confers Broad Cross-Reactive Immunity. Infect. Immun. 72: 338-344 [Abstract] [Full Text]  
  • Liu, X. S., Xu, Y., Hardy, L., Khammanivong, V., Zhao, W., Fernando, G. J. P., Leggatt, G. R., Frazer, I. H. (2003). IL-10 Mediates Suppression of the CD8 T Cell IFN-{gamma} Response to a Novel Viral Epitope in a Primed Host. J. Immunol. 171: 4765-4772 [Abstract] [Full Text]  
  • Dreher, D., Kok, M., Obregon, C., Kiama, S. G., Gehr, P., Nicod, L. P. (2002). Salmonella virulence factor SipB induces activation and release of IL-18 in human dendritic cells. J. Leukoc. Biol. 72: 743-751 [Abstract] [Full Text]  
  • Dreher, D., Kok, M., Cochand, L., Gitahi Kiama, S., Gehr, P., Pechère, J.-C., Nicod, L. P. (2001). Genetic background of attenuated Salmonella typhimurium has profound influence on infection and cytokine patterns in human dendritic cells. J. Leukoc. Biol. 69: 583-589 [Abstract] [Full Text]  
  • Niedergang, F., Sirard, J.-C., Blanc, C. T., Kraehenbuhl, J.-P. (2000). Entry and survival of Salmonella typhimurium in dendritic cells and presentation of recombinant antigens do not require macrophage-specific virulence factors. Proc. Natl. Acad. Sci. USA 97: 14650-14655 [Abstract] [Full Text]  
  • Chen, H., Schifferli, D. M. (2000). Mucosal and Systemic Immune Responses to Chimeric Fimbriae Expressed by Salmonella enterica Serovar Typhimurium Vaccine Strains. Infect. Immun. 68: 3129-3139 [Abstract] [Full Text]  
  • Huang, Y., Hajishengallis, G., Michalek, S. M. (2000). Construction and Characterization of a Salmonella enterica Serovar Typhimurium Clone Expressing a Salivary Adhesin of Streptococcus mutans under Control of the Anaerobically Inducible nirB Promoter. Infect. Immun. 68: 1549-1556 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»