Genetic Basis for Lipopolysaccharide O-Antigen Biosynthesis in Bordetellae

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Infection and Immunity, August 1999, p. 3763-3767, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genetic Basis for Lipopolysaccharide O-Antigen Biosynthesis in Bordetellae

Andrew Preston,¹^,^* Andrew G. Allen,¹ Joanna Cadisch,¹ Richard Thomas,¹ Kim Stevens,² Carol M. Churcher,² K. L. Badcock,² Julian Parkhill,² Bart Barrell,² and Duncan J. Maskell¹

Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES,¹ and Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA,² United Kingdom

Received 21 January 1999/Returned for modification 26 March 1999/Accepted 30 April 1999

Bordetella bronchiseptica and Bordetella parapertussis express a surface polysaccharide, attached to a lipopolysaccharide,which has been called O antigen. This structure is absent fromBordetella pertussis. We report the identification of a largegenetic locus in B. bronchiseptica and B. parapertussis that isrequired for O-antigen biosynthesis. The locus is replaced byan insertion sequence in B. pertussis, explaining the lack ofO-antigen biosynthesis in this species. The DNA sequence of theB. bronchiseptica locus has been determined and the presence of21 open reading frames has been revealed. We have ascribed putativefunctions to many of these open reading frames based on databasesearches. Mutations in the locus in B. bronchiseptica and B. parapertussisprevent O-antigen biosynthesis and provide tools for the studyof the role of O antigen in infections caused by thesebacteria.

^* Corresponding author. Mailing address: Centre for Veterinary Medicine, Department of Clinical Veterinary Medicine, Universityof Cambridge, Madingley Road, Cambridge CB3 0ES, United Kingdom.Phone: 44 1223 766233. Fax: 44 1223 337610. E-mail: ap243{at}mole.bio.cam.ac.uk.

Infection and Immunity, August 1999, p. 3763-3767, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

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