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Infection and Immunity, August 1999, p. 3763-3767, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genetic Basis for Lipopolysaccharide O-Antigen Biosynthesis in Bordetellae

Andrew Preston,1,* Andrew G. Allen,1 Joanna Cadisch,1 Richard Thomas,1 Kim Stevens,2 Carol M. Churcher,2 K. L. Badcock,2 Julian Parkhill,2 Bart Barrell,2 and Duncan J. Maskell1

Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES,1 and Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA,2 United Kingdom

Received 21 January 1999/Returned for modification 26 March 1999/Accepted 30 April 1999

Bordetella bronchiseptica and Bordetella parapertussis express a surface polysaccharide, attached to a lipopolysaccharide, which has been called O antigen. This structure is absent from Bordetella pertussis. We report the identification of a large genetic locus in B. bronchiseptica and B. parapertussis that is required for O-antigen biosynthesis. The locus is replaced by an insertion sequence in B. pertussis, explaining the lack of O-antigen biosynthesis in this species. The DNA sequence of the B. bronchiseptica locus has been determined and the presence of 21 open reading frames has been revealed. We have ascribed putative functions to many of these open reading frames based on database searches. Mutations in the locus in B. bronchiseptica and B. parapertussis prevent O-antigen biosynthesis and provide tools for the study of the role of O antigen in infections caused by these bacteria.

* Corresponding author. Mailing address: Centre for Veterinary Medicine, Department of Clinical Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, United Kingdom. Phone: 44 1223 766233. Fax: 44 1223 337610. E-mail: ap243{at}mole.bio.cam.ac.uk.

Infection and Immunity, August 1999, p. 3763-3767, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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