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Infection and Immunity, August 1999, p. 3763-3767, Vol. 67, No. 8
Centre for Veterinary Science,
Received 21 January 1999/Returned for modification 26 March
1999/Accepted 30 April 1999
Bordetella bronchiseptica and Bordetella
parapertussis express a surface polysaccharide, attached to a
lipopolysaccharide, which has been called O antigen. This structure is
absent from Bordetella pertussis. We report the
identification of a large genetic locus in B. bronchiseptica and B. parapertussis that is required
for O-antigen biosynthesis. The locus is replaced by an insertion
sequence in B. pertussis, explaining the lack of O-antigen
biosynthesis in this species. The DNA sequence of the B. bronchiseptica locus has been determined and the presence of 21 open reading frames has been revealed. We have ascribed putative functions to many of these open reading frames based on database searches. Mutations in the locus in B. bronchiseptica and
B. parapertussis prevent O-antigen biosynthesis and provide
tools for the study of the role of O antigen in infections caused by
these bacteria.
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Genetic Basis for Lipopolysaccharide O-Antigen
Biosynthesis in Bordetellae
*
Corresponding author. Mailing address: Centre for
Veterinary Medicine, Department of Clinical Veterinary Medicine,
University of Cambridge, Madingley Road, Cambridge CB3 0ES, United
Kingdom. Phone: 44 1223 766233. Fax: 44 1223 337610. E-mail:
ap243{at}mole.bio.cam.ac.uk.
Infection and Immunity, August 1999, p. 3763-3767, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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