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Infection and Immunity, August 1999, p. 4106-4111, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Purification and Properties of Proline-Rich Antimicrobial Peptides from Sheep and Goat Leukocytes

Olga Shamova,1,2 Kim A. Brogden,3 Chengquan Zhao,1 Tung Nguyen,1 Vladimir N. Kokryakov,1 and Robert I. Lehrer1,4,*

Department of Medicine1 and Molecular Biology Institute,4 UCLA School of Medicine, Los Angeles, California 90095; Department of General Pathology and Pathophysiology, Institute for Experimental Medicine, 19736 St. Petersburg, Russia2; and Respiratory and Neurologic Diseases Research Unit, Midwest Area National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 500103

Received 18 March 1999/Returned for modification 11 May 1999/Accepted 19 May 1999

We purified three proline-rich antimicrobial peptides from elastase-treated extracts of sheep and goat leukocytes and subjected two of them, OaBac5alpha and ChBac5, to detailed analysis. OaBac5alpha and ChBac5 were homologous to each other and to bovine Bac5. Both exhibited potent, broad-spectrum antimicrobial activity under low-concentration salt conditions. While the peptides remained active against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Listeria monocytogenes in 100 mM NaCl, they lost activity against Staphylococcus aureus and Candida albicans under these conditions. ChBac5 was shown to bind lipopolysaccharide, a property that could enhance its ability to kill gram-negative bacteria. Proline-rich Bac5 peptides are highly conserved in ruminants and may contribute significantly to their innate host defense mechanisms.

* Corresponding author. Mailing address: Department of Medicine, UCLA Center for the Health Sciences, 10833 LeConte Ave., Los Angeles, CA 90095-1690. Phone: (310) 825-5340. Fax: (310) 206-8766. E-mail: rlehrer{at}med1.medsch.ucla.edu.

Infection and Immunity, August 1999, p. 4106-4111, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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