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Infection and Immunity, August 1999, p. 4251-4255, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Detection of Als Proteins on the Cell Wall of Candida albicans in Murine Tissues

L. L. Hoyer,* J. Clevenger, J. E. Hecht, E. J. Ehrhart, and F. M. Pouletdagger

Department of Veterinary Pathobiology, University of Illinois, Urbana, Illinois

Received 23 February 1999/Returned for modification 19 March 1999/Accepted 11 May 1999

A murine model of disseminated candidiasis was utilized to determine whether Candida albicans Als proteins are produced in vivo. The kidneys, spleen, heart, liver, and lungs were collected from mice inoculated with one of three C. albicans strains (SC5314, B311, or WO-1). Immunohistochemical analysis of murine tissues by using a rabbit polyclonal anti-Als serum indicated that Als proteins were produced by each C. albicans cell in the tissues examined. Patterns of staining with the anti-Als serum were similar among the C. albicans strains tested. These data indicated that Als protein production was widespread in disseminated candidiasis and that, despite strain differences in ALS gene expression previously noted in vitro, Als protein production in vivo was similar among C. albicans strains. The extensive production of Als proteins in vivo and their presence on the C. albicans cell wall position these proteins well for a role in host-pathogen interaction.

* Corresponding author. Mailing address: Department of Veterinary Pathobiology, University of Illinois at Urbana-Champaign, 2522 VMBSB, 2001 S. Lincoln Ave., Urbana, IL 61802. Phone: (217) 333-5043. Fax: (217) 244-7421. E-mail: lhoyer{at}uiuc.edu.

dagger Present address: Schering-Plough Research Institute, Lafayette, NJ 07848.

Infection and Immunity, August 1999, p. 4251-4255, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.