This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Katz, J.
Right arrow Articles by Michalek, S. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Katz, J.
Right arrow Articles by Michalek, S. M.

 Previous Article  |  Next Article 

Infection and Immunity, September 1999, p. 4352-4359, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Host Responses to Recombinant Hemagglutinin B of Porphyromonas gingivalis in an Experimental Rat Model

Jannet Katz,2,* Kelley P. Black,1 and Suzanne M. Michalek1,2

Departments of Microbiology1 and Oral Biology,2 School of Dentistry, The University of Alabama at Birmingham, Birmingham, Alabama 35294

Received 22 February 1999/Returned for modification 26 April 1999/Accepted 5 June 1999

Porphyromonas gingivalis, a gram-negative, black-pigmented anaerobe, is among the microorganisms implicated in the etiology of adult periodontal disease. This bacterium possesses a number of factors, including hemagglutinins, of potential importance in virulence. Several hemagglutinin genes have been identified, cloned, and expressed in Escherichia coli. The purpose of this study was to characterize host responses to purified recombinant hemagglutinin B (rHag B), using the conventional Fischer rat as the experimental animal model. The effectiveness of immunization with rHag B on protection against experimental periodontal bone loss following infection with P. gingivalis was also evaluated. Groups of rats were immunized by the subcutaneous route with rHag B in complete Freund's adjuvant, immunized with rHag B and orally infected with P. gingivalis, nonimmunized and noninfected, or orally infected with P. gingivalis only. Serum and saliva samples were collected throughout the experiment and evaluated for serum immunoglobulin G (IgG) and IgM and salivary IgA antibody activity by enzyme-linked immunosorbent assay. No salivary IgA anti-Hag B activity was detected in the various groups of rats. A slight serum IgM response similar to that seen in preimmune samples was observed. Serum IgG antibody activity to Hag B was detected only in samples from rats immunized with rHag B. This response was primarily of the IgG1 and IgG2a subclasses, followed by IgG2b and low levels of IgG2c. Supernatants from rHag B-stimulated splenic lymphoid cell cultures from immunized rats contained high levels of gamma interferon, followed by interleukin-2 (IL-2), IL-10, and then IL-4. These results are consistent with the induction of T helper type 1 (Th1)- and Th2-like responses. Western blot analysis of sera derived from rHag B-immunized rats reacted with trichloroacetic acid (TCA) precipitates of P. gingivalis 33277, 381, A7A1-28, and W50, revealing a 50-kDa band reflective of Hag B. However, sera derived from rats immunized with P. gingivalis whole cells or from rats infected with P. gingivalis only did not react with rHag B but did react with TCA precipitates of P. gingivalis strains. Finally, radiographic measurements of periodontal bone loss indicated that rats immunized with rHag B had less bone loss than those infected with P. gingivalis only. These results demonstrate the effectiveness of purified rHag B in inducing a protective immune response and support the potential usefulness of this component of P. gingivalis in the development of a vaccine against adult periodontitis.

* Corresponding author. Mailing address: Department of Oral Biology, The University of Alabama at Birmingham, 845 South 19th St., BBRB 713/5, Birmingham, AL 35294-2170. Phone: (205) 934-2878. Fax: (205) 934-1426. E-mail: jenny_katz{at}micro.microbio.uab.edu.

Infection and Immunity, September 1999, p. 4352-4359, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Dietrich, D. E., Xiao, X., Dawson, D. V., Belanger, M., Xie, H., Progulske-Fox, A., Brogden, K. A. (2008). Human {alpha}- and {beta}-Defensins Bind to Immobilized Adhesins from Porphyromonas gingivalis. Infect. Immun. 76: 5714-5720 [Abstract] [Full Text]  
  • Teng, Y.-T.A. (2006). Protective and Destructive Immunity in the Periodontium: Part 2--T-cell-mediated Immunity in the Periodontium. JDR 85: 209-219 [Abstract] [Full Text]  
  • Han, X., Kawai, T., Eastcott, J. W., Taubman, M. A. (2006). Bacterial-Responsive B Lymphocytes Induce Periodontal Bone Resorption. J. Immunol. 176: 625-631 [Abstract] [Full Text]  
  • Song, H., Belanger, M., Whitlock, J., Kozarov, E., Progulske-Fox, A. (2005). Hemagglutinin B Is Involved in the Adherence of Porphyromonas gingivalis to Human Coronary Artery Endothelial Cells. Infect. Immun. 73: 7267-7273 [Abstract] [Full Text]  
  • Zhang, P., Martin, M., Michalek, S. M., Katz, J. (2005). Role of Mitogen-Activated Protein Kinases and NF-{kappa}B in the Regulation of Proinflammatory and Anti-Inflammatory Cytokines by Porphyromonas gingivalis Hemagglutinin B. Infect. Immun. 73: 3990-3998 [Abstract] [Full Text]  
  • Gibson, F. C. III, Gonzalez, D. A., Wong, J., Genco, C. A. (2004). Porphyromonas gingivalis-Specific Immunoglobulin G Prevents P. gingivalis-Elicited Oral Bone Loss in a Murine Model. Infect. Immun. 72: 2408-2411 [Abstract] [Full Text]  
  • Zhang, P., Martin, M., Yang, Q.-B., Michalek, S. M., Katz, J. (2004). Role of B7 Costimulatory Molecules in Immune Responses and T-Helper Cell Differentiation in Response to Recombinant HagB from Porphyromonas gingivalis. Infect. Immun. 72: 637-644 [Abstract] [Full Text]  
  • Gonzalez, D., Tzianabos, A. O., Genco, C. A., Gibson III, F. C. (2003). Immunization with Porphyromonas gingivalis Capsular Polysaccharide Prevents P. gingivalis-Elicited Oral Bone Loss in a Murine Model. Infect. Immun. 71: 2283-2287 [Abstract] [Full Text]  
  • Komerik, N., Nakanishi, H., MacRobert, A. J., Henderson, B., Speight, P., Wilson, M. (2003). In Vivo Killing of Porphyromonas gingivalis by Toluidine Blue-Mediated Photosensitization in an Animal Model. Antimicrob. Agents Chemother. 47: 932-940 [Abstract] [Full Text]  
  • DeCarlo, A. A., Huang, Y., Collyer, C. A., Langley, D. B., Katz, J. (2003). Feasibility of an HA2 Domain-Based Periodontitis Vaccine. Infect. Immun. 71: 562-566 [Abstract] [Full Text]  
  • Zhang, P., Jespersgaard, C., Lamberty-Mallory, L., Katz, J., Huang, Y., Hajishengallis, G., Michalek, S. M. (2002). Enhanced Immunogenicity of a Genetic Chimeric Protein Consisting of Two Virulence Antigens of Streptococcus mutans and Protection against Infection. Infect. Immun. 70: 6779-6787 [Abstract] [Full Text]  
  • Yang, Q.-B., Martin, M., Michalek, S. M., Katz, J. (2002). Mechanisms of Monophosphoryl Lipid A Augmentation of Host Responses to Recombinant HagB from Porphyromonas gingivalis. Infect. Immun. 70: 3557-3565 [Abstract] [Full Text]  
  • Rajapakse, P. S., O'Brien-Simpson, N. M., Slakeski, N., Hoffmann, B., Reynolds, E. C. (2002). Immunization with the RgpA-Kgp Proteinase-Adhesin Complexes of Porphyromonas gingivalis Protects against Periodontal Bone Loss in the Rat Periodontitis Model. Infect. Immun. 70: 2480-2486 [Abstract] [Full Text]  
  • Gibson, F. C. III, Genco, C. A. (2001). Prevention of Porphyromonas gingivalis-Induced Oral Bone Loss following Immunization with Gingipain R1. Infect. Immun. 69: 7959-7963 [Abstract] [Full Text]  
  • Yonezawa, H., Ishihara, K., Okuda, K. (2001). Arg-Gingipain A DNA Vaccine Induces Protective Immunity against Infection by Porphyromonas gingivalis in a Murine Model. Infect. Immun. 69: 2858-2864 [Abstract] [Full Text]  
  • Kohler, J. J., Pathangey, L., Hasona, A., Progulske-Fox, A., Brown, T. A. (2000). Long-Term Immunological Memory Induced by Recombinant Oral Salmonella Vaccine Vectors. Infect. Immun. 68: 4370-4373 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»