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Infection and Immunity, September 1999, p. 4525-4530, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Functional Studies of a Fibrinogen Binding Protein from Staphylococcus epidermidis

Lei Pei,1 Marco Palma,1 Martin Nilsson,2 Bengt Guss,2 and Jan-Ingmar Flock1,*

Department of Immunology, Microbiology, Pathology, and Infectious Diseases, Karolinska Institutet, Huddinge University Hospital, F82, S-141 86 Huddinge,1 and Department of Microbiology, Swedish University of Agricultural Sciences, S-75007 Uppsala,2 Sweden

Received 2 February 1999/Returned for modification 6 April 1999/Accepted 17 June 1999

A gene encoding a fibrinogen binding protein from Staphylococcus epidermidis was previously cloned, and the nucleotide sequence was determined. A portion of the gene encompassing the fibrinogen binding domain has now been subcloned in an expression-fusion vector. The fusion protein can bind to fibrinogen in a capture enzyme-linked immunosorbent assay and can be purified by fibrinogen affinity chromatography. This protein can completely inhibit the adherence of S. epidermidis to immobilized fibrinogen, suggesting that the adherence of S. epidermidis to fibrinogen is mainly due to this protein. Antibodies against this fibrinogen binding protein were also found to efficiently block the adherence of S. epidermidis to immobilized fibrinogen. Despite homology with clumping factors A and B from S. aureus (cell surface-associated proteins binding to fibrinogen), binding involved the beta  chain of fibrinogen rather than the gamma  chain, as in clumping factor A.

* Corresponding author. Mailing address: Department of Immunology, Microbiology, Pathology, and Infectious Diseases, Karolinska Institutet, Huddinge University Hospital, F82, S-141 86 Huddinge, Sweden. Phone: 46 8 58581169. Fax: 46 8 7113918. E-mail: jan-ingmar.flock{at}impi.ki.se.

Infection and Immunity, September 1999, p. 4525-4530, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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