Assessment of Immunity to Mycobacterial Infection with Luciferase Reporter Constructs

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Infection and Immunity, September 1999, p. 4586-4593, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Assessment of Immunity to Mycobacterial Infection with Luciferase Reporter Constructs

Valerie A. Snewin,^* Marie-Pierre Gares, Peadar Ó Gaora, Zahra Hasan, Ivor N. Brown, and Douglas B. Young

Department of Infectious Diseases and Microbiology, Imperial College School of Medicine, St. Mary's Campus, London W2 1PG, United Kingdom

Received 17 September 1998/Returned for modification 30 October 1998/Accepted 14 June 1999

Protective immunity to mycobacterial infection is incompletely understood but probably involves the coordinated interactionof multiple cell types and cytokines. With the aim of developingassays that might provide a surrogate measure of protective immunity,we have investigated the use of recombinant mycobacteria carryingluciferase reporter enzymes to assess the effectiveness of antimycobacterialimmunity in model systems. Measurement of luminescence was shownto provide a rapid and simple alternative to the counting of CFUas a means of monitoring mycobacterial viability. We describeoptimization of a luciferase reporter strain of Mycobacteriumtuberculosis and demonstrate its application for the study ofmycobacterial interactions with host cells in tissue culture andthe rapid assessment of vaccine efficacy in a murinemodel.

^* Corresponding author. Mailing address: Department of Infectious Diseases and Microbiology, Imperial College School of Medicine,St. Mary's Campus, Norfolk Place, London W2 1PG, United Kingdom.Phone: 44-171-594 3956. Fax: 44-171 262 6299. E-mail: v.snewin{at}ic.ac.uk.

Present address: Department of Microbiology, The Aga Khan University, Karachi 74800,Pakistan.

Infection and Immunity, September 1999, p. 4586-4593, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

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