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Infection and Immunity, September 1999, p. 4679-4688, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Isolation of Peptides That Mimic Epitopes on a Malarial Antigen from Random Peptide Libraries Displayed on Phage

Christopher G. Adda,1 Leann Tilley,1,2 Robin F. Anders,3 and Michael Foley1,2,*

Department of Biochemistry1 and the Cooperative Research Centre for Diagnostic Technologies,2 La Trobe University, Bundoora, and the Walter and Eliza Hall Institute of Medical Research, Melbourne,3 Victoria, Australia

Received 28 December 1998/Returned for modification 18 February 1999/Accepted 16 June 1999

The ring-infected erythrocyte surface antigen (RESA) is a dense-granule protein of Plasmodium falciparum which binds to the cytoskeletal structure of the erythrocyte after parasite invasion. It is currently under trial as a vaccine candidate. In an effort to characterize further the antibody responses to this antigen, we have panned two independent libraries of random peptides expressed on the surface of filamentous phage with a monoclonal antibody (MAb 18/2) against RESA. One library consisted of a potentially constrained 17-mer peptide fused with the gpVIII phage coat protein, and the other displayed an unconstrained 15-mer as a fusion with the minor phage coat protein gpIII. Several rounds of biopanning resulted in enrichment from both libraries clones that interacted specifically with MAb 18/2 in protein-blotting and enzyme-linked immunosorbent assay experiments. Nucleotide sequencing of the random oligonucleotide insert revealed a common predominant motif: (S/T)AVDD. Several other clones had related but degenerate motifs. Thus, a monoclonal antibody against a malarial antigen can select common mimotopes from different random peptide libraries. We envisage many uses for this technology in malaria research.

* Corresponding author. Mailing address: Department of Biochemistry, La Trobe University, Bundoora, Victoria 3083, Australia. Phone: 61-3-94792158. Fax: 61-3-9472467. E-mail: m.foley{at}latrobe.edu.au.

Infection and Immunity, September 1999, p. 4679-4688, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.