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Infection and Immunity, September 1999, p. 4847-4850, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Cell Surface-Exposed Tetanus Toxin Fragment C Produced by Recombinant Bacillus anthracis Protects against Tetanus Toxin

Stéphane Mesnage,* Martine Weber-Levy, Michel Haustant, Michèle Mock, and Agnès Fouet

Toxines et Pathogénie Bactériennes, URA 1858, Centre National de la Recherche Scientifique, Institut Pasteur, Paris, France

Received 25 March 1999/Returned for modification 6 May 1999/Accepted 25 May 1999

Bacillus anthracis, the causal agent of anthrax, synthesizes two surface layer (S-layer) proteins, EA1 and Sap, which account for 5 to 10% of total protein and are expressed in vivo. A recombinant B. anthracis strain was constructed by integrating into the chromosome a translational fusion harboring the DNA fragments encoding the cell wall-targeting domain of the S-layer protein EA1 and tetanus toxin fragment C (ToxC). This construct was expressed under the control of the promoter of the S-layer component gene. The hybrid protein was stably expressed on the cell surface of the bacterium. Mice were immunized with bacilli of the corresponding strain, and the hybrid protein elicited a humoral response to ToxC. This immune response was sufficient to protect mice against tetanus toxin challenge. Thus, the strategy developed in this study may make it possible to generate multivalent live veterinary vaccines, using the S-layer protein genes as a cell surface display system.

* Corresponding author. Mailing address: Toxines et Pathogénie Bactériennes, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris cédex 15, France. Phone: 33 1 45 68 86 54. Fax: 33 1 45 68 89 54. E-mail: smesnage{at}pasteur.fr.

Infection and Immunity, September 1999, p. 4847-4850, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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