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Infection and Immunity, September 1999, p. 4945-4949, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of a Third Metalloprotease Toxin Gene in Extraintestinal Isolates of Bacteroides fragilis

Gyung-Tae Chung,1 Augusto A. Franco,2 Shaoguang Wu,2 Gi-Eun Rhie,1 Rodney Cheng,2 Hee-Bok Oh,1 and Cynthia L. Sears2,*

Laboratory of Bacterial Toxins, Department of Microbiology, National Institute of Health, Seoul, Korea,1 and Divisions of Infectious Diseases and Gastroenterology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland2

Received 5 April 1999/Returned for modification 4 June 1999/Accepted 18 June 1999

To further understand the epidemiology of enterotoxigenic Bacteroides fragilis (ETBF), 89 extraintestinal B. fragilis strains from Seoul, Korea, were examined for secretion of B. fragilis toxin (BFT) by the HT29/C1 biologic assay and for the B. fragilis toxin gene (bft) by colony blot hybridization and PCR. Complete agreement between the three techniques was found. Overall, 34 B. fragilis strains (38%) were identified as ETBF. Eleven of the 34 ETBF strains (32%) expressed a new isoform of BFT (Korea-BFT). This new isoform is more related to BFT-2 than to BFT-1. Like BFT-1 and BFT-2, Korea-BFT cleaves E-cadherin, the zonula adherens protein.

* Corresponding author. Mailing address: Divisions of Infectious Diseases and Gastroenterology, Johns Hopkins University School of Medicine, Ross Bldg., Rm. 933, 720 Rutland Ave., Baltimore, MD 21205. Phone: (410) 955-9680. Fax: (410) 955-9677. E-mail: csears{at}welchlink.welch.jhu.edu.

Infection and Immunity, September 1999, p. 4945-4949, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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