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Infection and Immunity, September 1999, p. 4955-4959, Vol. 67, No. 9
Unité de Recherche en Vaccinologie,
Centre Hospitalier Universitaire de Québec et
Université Laval, Ste-Foy, Québec, Canada G1V 4G2
Received 5 May 1999/Returned for modification 26 May 1999/Accepted 15 June 1999
The cross-bactericidal and cross-protective activities of a
monoclonal antibody (MAb) named Me-7, which is directed against an
antigenically highly conserved epitope on the meningococcal NspA
protein, were studied. This MAb efficiently killed in vitro, in the
presence of rabbit or human serum, 13 of 14 meningococcal strains
tested, including 9 of 9, 2 of 3, and 2 of 2 strains of serotypes B, A,
and C, respectively. MAb Me-7 also significantly reduced by more than
75% the levels of bacteremia recorded for mice challenged with 10 of
11 meningococcal strains tested. Analysis of the predicted amino acid
sequence of the NspA protein from the meningococcal strain MCH88
(A:4:P1.10), which was not killed by MAb Me-7, indicated the presence
of an additional glutamine residue at position 73, compared to the
three other NspA sequences. The data presented in this study suggest
that antibodies directed against this highly conserved outer membrane
protein could protect against meningococcal infections.
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Bactericidal and Cross-Protective Activities of a Monoclonal
Antibody Directed against Neisseria meningitidis NspA
Outer Membrane Protein
*
Corresponding author. Mailing address: Unité de
Recherche en Vaccinologie, Centre Hospitalier Universitaire de
Québec, Pavillon CHUL, Édifice T-367, 2705 Blvd. Laurier,
Ste-Foy, Québec, Canada G1V 4G2. Phone: (418) 656-4141, ext.
6206. Fax: (418) 654-2280. E-mail:
Denis.Martin{at}crchul.ulaval.ca.
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