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Infection and Immunity, September 1999, p. 4968-4973, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Cloning of the Pasteurella haemolytica pomA Gene and Identification of Bovine Antibodies against PomA Surface Domains

Hui Zeng, Karamjeet Pandher,dagger and George L. Murphy*

Department of Anatomy, Pathology, and Pharmacology, College of Veterinary Medicine, Oklahoma State University, Stillwater, Oklahoma 74078

Received 11 March 1999/Returned for modification 12 April 1999/Accepted 11 June 1999

The gene (pomA) encoding PomA, an OmpA-like major outer membrane protein of the bovine respiratory pathogen Pasteurella haemolytica, was cloned, and its nucleotide sequence was determined. The deduced amino acid sequence of PomA has significant identity with the sequences of other OmpA family proteins. Absorption of three different bovine immune sera with whole P. haemolytica cells resulted in a reduction of bovine immunoglobulin G reactivity with recombinant PomA in Western immunoblots, suggesting the presence of antibodies against PomA surface domains.

* Corresponding author. Mailing address: Ambion, Inc., 2130 Woodward St., Austin, TX 78744-1832. Phone: 512-651-0200. Fax: 512-651-0201. E-mail: gmurphy{at}ambion.com.

dagger Present address: Abbott Laboratories, Dept. 047T, Bldg. AP3, Abbott Park, IL 60064.

Infection and Immunity, September 1999, p. 4968-4973, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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