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Infection and Immunity, January 2000, p. 303-309, Vol. 68, No. 1
0019-9567/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Seroreactivity to Chlamydia trachomatis Hsp10 Correlates with Severity of Human Genital Tract Disease

David LaVerda,1,dagger Lisa N. Albanese,1 Paul E. Ruther,2 Sandra G. Morrison,3 Richard P. Morrison,3 Kevin A. Ault,2 and Gerald I. Byrne1,*

Department of Medical Microbiology and Immunology, University of Wisconsin---Madison, Madison, Wisconsin 537061; Department of Obstetrics and Gynecology, University of Iowa Hospitals and Clinics, Iowa City, Iowa 522422; and Department of Microbiology, Montana State University, Bozeman, Montana 597173

Received 23 August 1999/Returned for modification 20 September 1999/Accepted 20 October 1999

We have identified the chlamydial heat shock protein Hsp10 as a potential correlate to the immunopathogenic process in women with tubal factor infertility (TFI). The human serologic response to chlamydial Hsp10, Hsp60, and major outer membrane protein (MOMP) was measured by enzyme-linked immunosorbent assay. Three populations of women were studied: uninfected controls (CU), acutely infected (AI) women, and women with TFI. Sera from women in the AI and TFI groups both recognized Hsp10 more frequently and at a higher overall level than sera from healthy uninfected controls. Moreover, the infertile women had significantly greater Hsp10 seroreactivity than acutely infected women, indicating a concomitant increase of Hsp10 recognition in populations with increasing levels of disease severity. Hsp60 reactivity showed a similar correlation in these populations, while MOMP reactivity peaked at the same level in both AI and TFI populations but did not increase with disease severity. Test populations were standardized by level of reactivity to formalin-fixed Chlamydia trachomatis elementary bodies (EBs) to address whether these associations were reflections of increased overall chlamydial exposure rather than a property specific to Hsp10. Associations between Hsp10 seropositivity and TFI were greater in the EB+ subgroup while associations among the EB- subgroup were diminished. When restricted to the EB+ subgroups, Hsp60 and MOMP responses in the TFI population did not increase significantly over the level of AI group responses. Thus, among women with similar exposure to chlamydiae, the serologic response to Hsp10 exhibited a stronger correlation with TFI than did the response to Hsp60 or MOMP. These findings support the hypothesis that the serological response to C. trachomatis heat shock proteins is associated with the severity of disease and identifies Hsp10 as an antigen recognized by a significant proportion of women with TFI.

* Corresponding author. Mailing address: University of Wisconsin---Madison, Department of Medical Microbiology and Immunology, 1300 University Ave., Madison, WI 53706. Phone: (608) 263-2494. Fax: (608) 265-0683. E-mail: gibyrne{at}facstaff.wisc.edu.

dagger Present address: The Maxwell Finland Institute for Infectious Diseases, Boston Medical Center, Boston, MA 02118.

Infection and Immunity, January 2000, p. 303-309, Vol. 68, No. 1
0019-9567/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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