This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by LaVerda, D. Articles by Byrne, G. I. Search for Related Content PubMed PubMed Citation Articles by LaVerda, D. Articles by Byrne, G. I.

Infection and Immunity, January 2000, p. 303-309, Vol. 68, No. 1
0019-9567/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Seroreactivity to Chlamydia trachomatis Hsp10 Correlates with Severity of Human Genital Tract Disease

David LaVerda,^1, Lisa N. Albanese,¹ Paul E. Ruther,² Sandra G. Morrison,³ Richard P. Morrison,³ Kevin A. Ault,² and Gerald I. Byrne^1,*

Department of Medical Microbiology and Immunology, University of WisconsinMadison, Madison, Wisconsin 53706¹; Department of Obstetrics and Gynecology, University of Iowa Hospitals and Clinics, Iowa City, Iowa 52242²; and Department of Microbiology, Montana State University, Bozeman, Montana 59717³

Received 23 August 1999/Returned for modification 20 September 1999/Accepted 20 October 1999

We have identified the chlamydial heat shock protein Hsp10 as a potential correlate to the immunopathogenic process in women with tubal factor infertility (TFI). The human serologic response to chlamydial Hsp10, Hsp60, and major outer membrane protein (MOMP) was measured by enzyme-linked immunosorbent assay. Three populations of women were studied: uninfected controls (CU), acutely infected (AI) women, and women with TFI. Sera from women in the AI and TFI groups both recognized Hsp10 more frequently and at a higher overall level than sera from healthy uninfected controls. Moreover, the infertile women had significantly greater Hsp10 seroreactivity than acutely infected women, indicating a concomitant increase of Hsp10 recognition in populations with increasing levels of disease severity. Hsp60 reactivity showed a similar correlation in these populations, while MOMP reactivity peaked at the same level in both AI and TFI populations but did not increase with disease severity. Test populations were standardized by level of reactivity to formalin-fixed Chlamydia trachomatis elementary bodies (EBs) to address whether these associations were reflections of increased overall chlamydial exposure rather than a property specific to Hsp10. Associations between Hsp10 seropositivity and TFI were greater in the EB⁺ subgroup while associations among the EB subgroup were diminished. When restricted to the EB⁺ subgroups, Hsp60 and MOMP responses in the TFI population did not increase significantly over the level of AI group responses. Thus, among women with similar exposure to chlamydiae, the serologic response to Hsp10 exhibited a stronger correlation with TFI than did the response to Hsp60 or MOMP. These findings support the hypothesis that the serological response to C. trachomatis heat shock proteins is associated with the severity of disease and identifies Hsp10 as an antigen recognized by a significant proportion of women with TFI.

---

^* Corresponding author. Mailing address: University of WisconsinMadison, Department of Medical Microbiology and Immunology, 1300 University Ave., Madison, WI 53706. Phone: (608) 263-2494. Fax: (608) 265-0683. E-mail: gibyrne{at}facstaff.wisc.edu.

Present address: The Maxwell Finland Institute for Infectious Diseases, Boston Medical Center, Boston, MA 02118.

---

Infection and Immunity, January 2000, p. 303-309, Vol. 68, No. 1
0019-9567/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

• LaRue, R. W., Dill, B. D., Giles, D. K., Whittimore, J. D., Raulston, J. E. (2007). Chlamydial Hsp60-2 Is Iron Responsive in Chlamydia trachomatis Serovar E-Infected Human Endometrial Epithelial Cells In Vitro. Infect. Immun. 75: 2374-2380 [Abstract] [Full Text]  
• Equils, O., Lu, D., Gatter, M., Witkin, S. S., Bertolotto, C., Arditi, M., McGregor, J. A., Simmons, C. F., Hobel, C. J. (2006). Chlamydia Heat Shock Protein 60 Induces Trophoblast Apoptosis through TLR4. J. Immunol. 177: 1257-1263 [Abstract] [Full Text]  
• Lyons, R.A., Saridogan, E., Djahanbakhch, O. (2006). The reproductive significance of human Fallopian tube cilia. Hum Reprod Update 12: 363-372 [Abstract] [Full Text]  
• Tiitinen, A., Surcel, H.-M., Halttunen, M., Birkelund, S., Bloigu, A., Christiansen, G., Koskela, P., Morrison, S.G., Morrison, R.P., Paavonen, J. (2006). Chlamydia trachomatis and chlamydial heat shock protein 60-specific antibody and cell-mediated responses predict tubal factor infertility. Hum Reprod 21: 1533-1538 [Abstract] [Full Text]  
• Sharma, J., Zhong, Y., Dong, F., Piper, J. M., Wang, G., Zhong, G. (2006). Profiling of Human Antibody Responses to Chlamydia trachomatis Urogenital Tract Infection Using Microplates Arrayed with 156 Chlamydial Fusion Proteins. Infect. Immun. 74: 1490-1499 [Abstract] [Full Text]  
• Karinen, L., Pouta, A., Bloigu, A., Koskela, P., Paldanius, M., Leinonen, M., Saikku, P., Jervelin, M.-R., Hartikainen, A.-L. (2005). Serum C-reactive Protein and Chlamydia trachomatis Antibodies in Preterm Delivery. Obstet Gynecol 106: 73-80 [Abstract] [Full Text]  
• Higgins, D. P., Hemsley, S., Canfield, P. J. (2005). Association of Uterine and Salpingeal Fibrosis with Chlamydial Hsp60 and Hsp10 Antigen-Specific Antibodies in Chlamydia-Infected Koalas. CVI 12: 632-639 [Abstract] [Full Text]  
• Sharma, J., Bosnic, A. M., Piper, J. M., Zhong, G. (2004). Human Antibody Responses to a Chlamydia-Secreted Protease Factor. Infect. Immun. 72: 7164-7171 [Abstract] [Full Text]  
• Maxion, H. K., Liu, W., Chang, M.-H., Kelly, K. A. (2004). The Infecting Dose of Chlamydia muridarum Modulates the Innate Immune Response and Ascending Infection. Infect. Immun. 72: 6330-6340 [Abstract] [Full Text]  
• Wilson, A. C., Tan, M. (2004). Stress Response Gene Regulation in Chlamydia Is Dependent on HrcA-CIRCE Interactions. J. Bacteriol. 186: 3384-3391 [Abstract] [Full Text]  
• Lichtenwalner, A. B., Patton, D. L., Van Voorhis, W. C., Sweeney, Y. T. C., Kuo, C.-C. (2004). Heat Shock Protein 60 Is the Major Antigen Which Stimulates Delayed-Type Hypersensitivity Reaction in the Macaque Model of Chlamydia trachomatis Salpingitis. Infect. Immun. 72: 1159-1161 [Abstract] [Full Text]  
• Kalayoglu, M. V., Galvan, C., Mahdi, O. S., Byrne, G. I., Mansour, S. (2003). Serological Association Between Chlamydia pneumoniae Infection and Age-Related Macular Degeneration. Arch Ophthalmol 121: 478-482 [Abstract] [Full Text]  
• Mahdi, O. S., Horne, B. D., Mullen, K., Muhlestein, J. B., Byrne, G. I. (2002). Serum Immunoglobulin G Antibodies to Chlamydial Heat Shock Protein 60 but Not to Human and Bacterial Homologs Are Associated With Coronary Artery Disease. Circulation 106: 1659-1663 [Abstract] [Full Text]  
• Kinnunen, A.H., Surcel, H-M., Lehtinen, M., Karhukorpi, J., Tiitinen, A., Halttunen, M., Bloigu, A., Morrison, R.P., Karttunen, R., Paavonen, J. (2002). HLA DQ alleles and interleukin-10 polymorphism associated with Chlamydia trachomatis-related tubal factor infertility: a case-control study. Hum Reprod 17: 2073-2078 [Abstract] [Full Text]  
• Eggert-Kruse, W., Neuer, A., Clussmann, C., Boit, R., Geissler, W., Rohr, G., Strowitzki, T. (2002). Seminal antibodies to human 60kd heat shock protein (HSP 60) in male partners of subfertile couples. Hum Reprod 17: 726-735 [Abstract] [Full Text]  
• Bulut, Y., Faure, E., Thomas, L., Karahashi, H., S. Michelsen, K., Equils, O., Morrison, S. G., Morrison, R. P., Arditi, M. (2002). Chlamydial Heat Shock Protein 60 Activates Macrophages and Endothelial Cells Through Toll-Like Receptor 4 and MD2 in a MyD88-Dependent Pathway. J. Immunol. 168: 1435-1440 [Abstract] [Full Text]  
• Bas, S., Muzzin, P., Ninet, B., Bornand, J. E., Scieux, C., Vischer, T. L. (2001). Chlamydial Serology: Comparative Diagnostic Value of Immunoblotting, Microimmunofluorescence Test, and Immunoassays Using Different Recombinant Proteins as Antigens. J. Clin. Microbiol. 39: 1368-1377 [Abstract] [Full Text]  
• Sasu, S., LaVerda, D., Qureshi, N., Golenbock, D. T., Beasley, D. (2001). Chlamydia pneumoniae and Chlamydial Heat Shock Protein 60 Stimulate Proliferation of Human Vascular Smooth Muscle Cells via Toll-Like Receptor 4 and p44/p42 Mitogen-Activated Protein Kinase Activation. Circ. Res. 89: 244-250 [Abstract] [Full Text]