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Infection and Immunity, January 2000, p. 342-351, Vol. 68, No. 1
0019-9567/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
In Vitro Brucella suis Infection
Prevents the Programmed Cell Death of Human Monocytic Cells
Antoine
Gross,
Annie
Terraza,
Safia
Ouahrani-Bettache,
Jean-Pierre
Liautard, and
Jacques
Dornand*
INSERM U431, IFR Eugène Bataillon,
Université de Montpellier II, 34095 Montpellier Cedex 5, France
Received 3 June 1999/Returned for modification 29 July
1999/Accepted 29 September 1999
During the complex interaction between an infectious agent and a
host organism, the pathogen can interfere with the host cell's programmed death to its own benefit. Induction or prevention of host
cell apoptosis appears to be a critical step for determining the
infection outcome. Members of the gram-negative bacterial genus
Brucella are intracellular pathogens which preferentially invade monocytic cells and develop within these cells. We investigated the effect of Brucella suis infection on apoptosis of human
monocytic phagocytes. The present study provides evidence that
Brucella infection inhibited spontaneously occurring
apoptosis in human monocytes. Prevention of monocyte apoptosis was not
mediated by Brucella lipopolysaccharide and required
bacterial survival within infected cells. Both invaded and noninvaded
cells were protected, indicating that soluble mediators released during
infection were involved in the phenomenon. Analysis of
Brucella-infected monocytes revealed specific
overexpression of the A1 gene, a member of the bcl-2 family implicated in the survival of hematopoietic
cells. Brucella infection also rendered macrophage-like
cells resistant to Fas ligand- or gamma interferon-induced apoptosis,
suggesting that Brucella infection protected host cells
from several cytotoxic processes occurring at different steps of the
immune response. The present data clearly show that Brucella
suis modulated the monocyte/macrophage's apoptotic response to
the advantage of the pathogen, thus preventing host cell elimination.
This might represent a strategy for Brucella development in
infected hosts.
*
Corresponding author. Mailing address: INSERM U431, IFR
Eugène Bataillon, Université de Montpellier II CC100, Place
Eugène Bataillon, 34095 Montpellier Cedex 5, France. Phone: 33 (0)4 67144244. Fax: 33 (0)4 67143338. E-mail:
dornand{at}crit.univ-montp2.fr.
Infection and Immunity, January 2000, p. 342-351, Vol. 68, No. 1
0019-9567/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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