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Infection and Immunity, January 2000, p. 407-410, Vol. 68, No. 1
0019-9567/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Toxoplasma gondii Infection Induces Gene Expression and Secretion of Interleukin 1 (IL-1), IL-6, Granulocyte-Macrophage Colony-Stimulating Factor, and Intercellular Adhesion Molecule 1 by Human Retinal Pigment Epithelial Cells

Chandrasekharam N. Nagineni,1 Barbara Detrick,2,dagger and John J. Hooks1,*

Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892,1 and Department of Pathology, The George Washington University Medical Center, Washington, D.C. 200372

Received 19 July 1999/Returned for modification 9 September 1999/Accepted 11 October 1999

We have used human retinal pigment epithelial (HRPE) cultures to investigate the primary cellular responses of retinal resident cells to intracellular Toxoplasma gondii replication. At 4 days postinoculation, when all of the cells were infected, the secretion of interleukin 1beta (IL-1beta ), IL-6, granulocyte-macrophage colony-stimulating factor (GM-CSF), and intercellular adhesion molecule 1 (ICAM-1) was augmented by 23-, 10-, 8-, and 5-fold, respectively, over the control. Northern and reverse transcriptase PCR analyses showed significant upregulation of steady-state levels of mRNA for IL-1beta , IL-6, GM-CSF, and ICAM-1. The secretion of these molecules by HRPE cells may play a critical immunoregulatory role in the pathophysiological processes associated with T. gondii-induced retinochoroiditis.

* Corresponding author. Mailing address: Immunology and Virology Section, Laboratory of Immunology, National Eye Institute, Building 10, Room 6N 228, National Institutes of Health, Bethesda, MD 20892. Phone: (301) 496-6578. Fax: (301) 480-2988. E-mail: jjhooks{at}helix.nih.gov.

dagger Present address: Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Md.

Infection and Immunity, January 2000, p. 407-410, Vol. 68, No. 1
0019-9567/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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