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Infection and Immunity, January 2000, p. 415-419, Vol. 68, No. 1
0019-9567/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Isolation of Bacteroides fragilis Mutants with In Vivo Growth Defects by Using Tn4400', a Modified Tn4400 Transposition System, and a New Screening Method

Yixin P. Tang and Michael H. Malamy*

Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111

Received 5 March 1999/Returned for modification 14 May 1999/Accepted 5 June 1999

A modified version of the Bacteroides fragilis transposon Tn4400, designated Tn4400', enabling rapid isolation and analysis of B. fragilis mutants has been constructed. To identify potential virulence factors, Tn4400'-generated mutants were screened by a new method; this resulted in the isolation of 21 mutant strains with impaired growth characteristics on tissue culture monolayers but normal growth in rich medium anaerobically.

* Corresponding author. Mailing address: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Ave., Boston, MA 02111. Phone: (617) 636-6756. Fax: (617) 636-0337. E-mail: mmalamy1{at}opal.tufts.edu.

Infection and Immunity, January 2000, p. 415-419, Vol. 68, No. 1
0019-9567/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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