This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Laine, V. J. O.
Right arrow Articles by Nevalainen, T. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Laine, V. J. O.
Right arrow Articles by Nevalainen, T. J.

 Previous Article  |  Next Article 

Infection and Immunity, January 2000, p. 87-92, Vol. 68, No. 1
0019-9567/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Resistance of Transgenic Mice Expressing Human Group II Phospholipase A2 to Escherichia coli Infection

V. Jukka O. Laine,1,* David S. Grass,2 and Timo J. Nevalainen1

Department of Pathology, University of Turku, 20520 Turku, Finland,1 and Chrysalis DNX Transgenics, Princeton, New Jersey 085402

Received 10 June 1999/Returned for modification 11 August 1999/Accepted 20 October 1999

Group II phospholipase A2 (PLA2) is a newly recognized antibacterial acute-phase protein. Recently we observed that transgenic mice expressing group II PLA2 (PLA2+ mice) were able to resist experimental Staphylococcus aureus infection by killing the bacteria, as indicated by improved survival and by the small numbers of live bacteria in their tissues (V. J. O. Laine, D. S. Grass, and T. J. Nevalainen, J. Immunol. 162:7402-7408, 1999). To establish the role of group II PLA2 in Escherichia coli infection, the host responses of PLA2+ mice and their PLA2-deficient C57BL/6J littermates (PLA2- mice) were studied after intraperitoneal administration of E. coli. The levels of group II PLA2 in sera of PLA2+ mice increased after the administration of E. coli, and the concentration of group II PLA2 correlated significantly with the catalytic activity of PLA2 in serum. PLA2+ mice showed lower rates of mortality and less bacterial growth in peritoneal lavage fluid, blood, and spleen and liver tissues than PLA2- mice. Unlike the observations with staphylococcal infection, serum and peritoneal lavage fluid did not inhibit the growth of E. coli in vitro. The results indicate that expression of the group II PLA2 transgene improves the host defense of mice against E. coli infection.


* Corresponding author. Mailing address: Department of Pathology, University of Turku, Kiinamyllynkatu 10, FIN-20520 Turku, Finland. Phone: (358) 2-261 2670. Fax: (358) 2-333 7459. E-mail: vellai{at}utu.fi.


Infection and Immunity, January 2000, p. 87-92, Vol. 68, No. 1
0019-9567/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Hurley, B. P., McCormick, B. A. (2008). Multiple Roles of Phospholipase A2 during Lung Infection and Inflammation. Infect. Immun. 76: 2259-2272 [Full Text]  
  • Koprivnjak, T., Weidenmaier, C., Peschel, A., Weiss, J. P. (2008). Wall Teichoic Acid Deficiency in Staphylococcus aureus Confers Selective Resistance to Mammalian Group IIA Phospholipase A2 and Human {beta}-Defensin 3. Infect. Immun. 76: 2169-2176 [Abstract] [Full Text]  
  • Kim, J.-O., Chakrabarti, B. K., Guha-Niyogi, A., Louder, M. K., Mascola, J. R., Ganesh, L., Nabel, G. J. (2007). Lysis of Human Immunodeficiency Virus Type 1 by a Specific Secreted Human Phospholipase A2. J. Virol. 81: 1444-1450 [Abstract] [Full Text]  
  • Kirschnek, S., Gulbins, E. (2006). Phospholipase A2 Functions in Pseudomonas aeruginosa- Induced Apoptosis. Infect. Immun. 74: 850-860 [Abstract] [Full Text]  
  • Wootton, P. T.E., Drenos, F., Cooper, J. A., Thompson, S. R., Stephens, J. W., Hurt-Camejo, E., Wiklund, O., Humphries, S. E., Talmud, P. J. (2006). Tagging-SNP haplotype analysis of the secretory PLA2IIa gene PLA2G2A shows strong association with serum levels of sPLA2IIa: results from the UDACS study. Hum Mol Genet 15: 355-361 [Abstract] [Full Text]  
  • Piris-Gimenez, A., Paya, M., Lambeau, G., Chignard, M., Mock, M., Touqui, L., Goossens, P. L. (2005). In Vivo Protective Role of Human Group IIA Phospholipase A2 against Experimental Anthrax. J. Immunol. 175: 6786-6791 [Abstract] [Full Text]  
  • Femling, J. K., Nauseef, W. M., Weiss, J. P. (2005). Synergy between Extracellular Group IIA Phospholipase A2 and Phagocyte NADPH Oxidase in Digestion of Phospholipids of Staphylococcus aureus Ingested by Human Neutrophils. J. Immunol. 175: 4653-4661 [Abstract] [Full Text]  
  • Tan, X.-X., Actor, J. K., Chen, Y. (2005). Peptide Nucleic Acid Antisense Oligomer as a Therapeutic Strategy against Bacterial Infection: Proof of Principle Using Mouse Intraperitoneal Infection. Antimicrob. Agents Chemother. 49: 3203-3207 [Abstract] [Full Text]  
  • Dubouix, A., Campanac, C., Fauvel, J., Simon, M.-F., Salles, J.-P., Roques, C., Chap, H., Marty, N. (2003). Bactericidal properties of group IIa secreted phospholipase A2 against Pseudomonas aeruginosa clinical isolates. J Med Microbiol 52: 1039-1045 [Abstract] [Full Text]  
  • Tietge, U. J. F., Maugeais, C., Cain, W., Rader, D. J. (2003). Acute inflammation increases selective uptake of HDL cholesteryl esters into adrenals of mice overexpressing human sPLA2. Am. J. Physiol. Endocrinol. Metab. 285: E403-E411 [Abstract] [Full Text]  
  • Park, D.-W., Kim, J.-R., Kim, S.-Y., Sonn, J.-K., Bang, O.-S., Kang, S.-S., Kim, J.-H., Baek, S.-H. (2003). Akt as a Mediator of Secretory Phospholipase A2 Receptor-Involved Inducible Nitric Oxide Synthase Expression. J. Immunol. 170: 2093-2099 [Abstract] [Full Text]  
  • Koprivnjak, T., Peschel, A., Gelb, M. H., Liang, N. S., Weiss, J. P. (2002). Role of Charge Properties of Bacterial Envelope in Bactericidal Action of Human Group IIA Phospholipase A2 against Staphylococcus aureus. J. Biol. Chem. 277: 47636-47644 [Abstract] [Full Text]  
  • Koduri, R. S., Gronroos, J. O., Laine, V. J. O., Le Calvez, C., Lambeau, G., Nevalainen, T. J., Gelb, M. H. (2002). Bactericidal Properties of Human and Murine Groups I, II, V, X, and XII Secreted Phospholipases A2. J. Biol. Chem. 277: 5849-5857 [Abstract] [Full Text]  
  • Cummings, B. S., McHowat, J., Schnellmann, R. G. (2000). Phospholipase A2s in Cell Injury and Death. J. Pharmacol. Exp. Ther. 294: 793-799 [Abstract] [Full Text]  
  • Murakami, M., Koduri, R. S., Enomoto, A., Shimbara, S., Seki, M., Yoshihara, K., Singer, A., Valentin, E., Ghomashchi, F., Lambeau, G., Gelb, M. H., Kudo, I. (2001). Distinct Arachidonate-releasing Functions of Mammalian Secreted Phospholipase A2s in Human Embryonic Kidney 293 and Rat Mastocytoma RBL-2H3 Cells through Heparan Sulfate Shuttling and External Plasma Membrane Mechanisms. J. Biol. Chem. 276: 10083-10096 [Abstract] [Full Text]