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Infection and Immunity, October 2000, p. 5517-5524, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Bacterial Immunoglobulin Superantigen Proteins A and L Activate Human Heart Mast Cells by Interacting with Immunoglobulin E

Arturo Genovese,1 Jean-Pierre Bouvet,2 Giovanni Florio,1 Bärbel Lamparter-Schummert,3 Lars Björck,4 and Gianni Marone1,*

Division of Clinical Immunology and Allergy, University of Naples Federico II, Naples, Italy1; Unité d'Immunopathologie Humaine, Institut National de la Santé et de la Recherche Médicale U450, Hôpital Broussais, Paris, France2; Department of Anesthesiology, Deutsches Herzzentrum Berlin, Berlin, Germany3; and Department of Cell and Molecular Biology, University of Lund, Lund, Sweden4

Received 17 May 2000/Accepted 7 July 2000

Human heart mast cells (HHMC) have been identified in heart tissue, perivascularly, and in the intima of coronary arteries. In vitro activation of isolated HHMC induces the release of vasoactive and proinflammatory mediators (histamine, tryptase, and cysteinyl leukotriene C4 [LTC4]). We investigated the effects of several bacterial proteins on HHMC activation in vitro. HHMC released histamine, tryptase, and LTC4 in response to Staphylococcus aureus Cowan 1 and the immunoglobulin (Ig)-binding protein A, but not to S. aureus Wood 46, which does not synthesize protein A. The effect of protein A was inhibited by preincubation with monoclonal IgM VH3+. Some strains of Peptostreptococcus magnus express an Ig light chain-binding surface protein called protein L. Such bacteria and soluble protein L stimulated the release of preformed and newly synthesized mediators from HHMC. Preincubation of HHMC with either protein A or protein L resulted in complete cross-desensitization to a subsequent challenge with the heterologous stimulus or anti-IgE. Monoclonal IgE (kappa  chains) blocked protein L-induced release, whereas IgE (lambda  chains) had no effect. Streptococcal protein G, formyl-containing tripeptide, and pepstatin A did not activate HHMC. Bacterial products protein A and protein L and intact bacteria (S. aureus and P. magnus) activate HHMC by acting as Ig superantigens.

* Corresponding author. Mailing address: Divisione di Immunologia Clinica e Allergologia, Università di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, Italy. Phone: 39-081-7707492. Fax: 39-081-7462271. E-mail: marone{at}unina.it.

Infection and Immunity, October 2000, p. 5517-5524, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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