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Infection and Immunity, October 2000, p. 5581-5586, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Recombinant Bovine Interleukin-1beta Amplifies the Effects of Partially Purified Pasteurella haemolytica Leukotoxin on Bovine Neutrophils in a beta 2-Integrin-Dependent Manner

F. Leite,1 J. F. Brown,1 M. J. Sylte,1 R. E. Briggs,2 and C. J. Czuprynski1,*

Department of Pathobiological Sciences, University of Wisconsin School of Veterinary Medicine, Madison, Wisconsin,1 and National Animal Disease Center, USDA Agricultural Research Service, Ames, Iowa2

Received 10 April 2000/Returned for modification 27 May 2000/Accepted 4 July 2000

The influx and death of polymorphonuclear leukocytes within the infected lung are hallmarks of bovine pasteurellosis. Recent reports have shown that the Pasteurella haemolytica leukotoxin (LKT) and other RTX toxins bind beta 2-integrins on target cells. In this study we demonstrate that exposure of bovine neutrophils to recombinant bovine interleukin-1beta upregulates beta 2-integrins (CD11a/CD18), which in turn enhance the binding and amplify the biological effects of partially purified LKT on these cells. LKT binding and cytotoxicity were inhibited by addition of an anti-integrin antibody (CD11a/CD18). These findings help to clarify the early events that occur in bovine pasteurellosis and support the hypothesis that inflammatory mediators might increase the severity of pasteurellosis by causing upregulation of beta 2-integrins that serve as an LKT receptor on bovine neutrophils.

* Corresponding author. Mailing address: Department of Pathobiological Sciences, School of Veterinary Medicine, 2015 Linden Drive West, Madison, WI 53706. Phone: (608) 262-8102. Fax: (608) 262-8102. E-mail: czuprync{at}svm.vetmed.wisc.edu.

Infection and Immunity, October 2000, p. 5581-5586, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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