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Infection and Immunity, October 2000, p. 5587-5594, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Expression of Mucosal Homing Receptor alpha 4beta 7 Is Associated with Enhanced Migration to the Chlamydia-Infected Murine Genital Mucosa In Vivo

Raymond A. Hawkins,1 Roger G. Rank,1 and Kathleen A. Kelly2,*

Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas,1 and Department of Pathology and Laboratory Medicine, UCLA Medical Center, Los Angeles, California2

Received 16 March 2000/Returned for modification 3 May 2000/Accepted 7 July 2000

The CD4 T helper cell type 1 (Th1) response is essential for the resolution of chlamydial genital infection in mice. However, not all Th1 clones are equally protective in eradicating the infection. Since oral immunization regimens produce protective immunity, we evaluated the role of the mucosa-associated homing receptor, alpha 4beta 7, in trafficking to the genital mucosa. Using a panel of CD4, Th1 cell lines and clones, we compared the lymphocyte homing patterns of a Chlamydia-specific, protective clone (P-MoPn), a nonprotective clone (N-MoPn), and a keyhole limpet hemocyanin (KLH)-specific cell line (KLH-1). T cells were labeled with the fluorescent dye PKH-26, adoptively transferred into Chlamydia-infected mice, and monitored at different time points throughout the course of a genital infection. We found that clones P-MoPn and N-MoPn migrated to similar extents to the genital tract and in significantly greater numbers than the KLH-specific T-cell line. Both clones and the KLH-1 line expressed similar levels of the adhesion molecules alpha 4, beta 1, CD44, and CD11a. However, clones P-MoPn and N-MoPn expressed higher levels of the mucosal homing receptor, alpha 4beta 7. Also, clones P-MoPn and N-MoPn but not the KLH-1 line migrated to the mesenteric lymph node, suggesting a mucosal recirculation pattern. Moreover, blocking alpha 4beta 7 adhesion interaction in vivo significantly reduced the recruitment of P-MoPn but not KLH-1 to the genital tract. These findings show that the mucosal homing receptor alpha 4beta 7 is utilized by a subset of CD4 cells during migration to the Chlamydia-infected genital tract.

* Corresponding author. Mailing address: UCLA Medical Center, Department of Pathology and Laboratory Medicine, 10833 Le Conte Ave., Mailroom A2-179 CHS, Los Angeles, CA 90095-1732. Phone: (310) 206-5562. Fax: (310) 794-4863. E-mail: kkelly{at}mednet.uams.edu.

Infection and Immunity, October 2000, p. 5587-5594, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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