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Infection and Immunity, October 2000, p. 5673-5678, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

alpha vbeta 3 Integrin and Bacterial Lipopolysaccharide Are Involved in Coxiella burnetii-Stimulated Production of Tumor Necrosis Factor by Human Monocytes

Jérôme Dellacasagrande,1 Eric Ghigo,1 Sarah Machergui-El , Hammami,1 Rudolf Toman,2 Didier Raoult,1 Christian Capo,1 and Jean-Louis Mege1,*

Unité des Rickettsies, CNRS UPRESA 6020, Faculté de Médecine, Université de la Méditerranée, 13385 Marseille Cedex 05, France,1 and Department of Rickettsiology and Chlamydiology, Institute of Virology, Slovak Academy of Sciences, 842 45 Bratislava, Slovak Republic2

Received 10 April 2000/Accepted 26 June 2000

Coxiella burnetii, the agent of Q fever, enters human monocytes through alpha vbeta 3 integrin and survives inside host cells. In addition, C. burnetii stimulates the synthesis of inflammatory cytokines including tumor necrosis factor (TNF) by monocytes. We studied the role of the interaction of C. burnetii with THP-1 monocytes in TNF production. TNF transcripts and TNF release reached maximum values within 4 h. Almost all monocytes bound C. burnetii after 4 h, while the percentage of phagocytosing monocytes did not exceed 20%. Cytochalasin D, which prevented the uptake of C. burnetii without interfering with its binding, did not affect the expression of TNF mRNA. Thus, bacterial adherence, but not phagocytosis, is necessary for TNF production by monocytes. The monocyte alpha vbeta 3 integrin was involved in TNF synthesis since peptides containing RGD sequences and blocking antibodies against alpha vbeta 3 integrin inhibited TNF transcripts induced by C. burnetii. Nevertheless, the cross-linking of alpha vbeta 3 integrin by specific antibodies was not sufficient to induce TNF synthesis. The signal delivered by C. burnetii was triggered by bacterial lipopolysaccharide (LPS). Polymyxin B inhibited the TNF production stimulated by C. burnetii, and soluble LPS isolated from C. burnetii largely mimicked viable bacteria. On the other hand, avirulent variants of C. burnetii induced TNF production through an increased binding to monocytes rather than through the potency of their LPS. We suggest that the adherence of C. burnetii to monocytes via alpha vbeta 3 integrin enables surface LPS to stimulate TNF production in THP-1 monocytes.

* Corresponding author. Mailing address: Unité des Rickettsies, CNRS UPRESA 6020, Faculté de Médecine, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France. Phone: (33) 4 91 32 43 75. Fax: (33) 4 91 38 77 72. E-mail: Jean-Louis.Mege{at}medecine.univ-mrs.fr.

Infection and Immunity, October 2000, p. 5673-5678, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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