Previous Article | Next Article 
Infection and Immunity, October 2000, p. 5679-5689, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Sequence Polymorphism, Predicted Secondary Structures, and
Surface-Exposed Conformational Epitopes of Campylobacter
Major Outer Membrane Protein
Qijing
Zhang,1,2,*
Jerrel C.
Meitzler,1
Shouxiong
Huang,1,2 and
Teresa
Morishita2
Food Animal Health Research
Program,1 and Department of Veterinary
Preventive Medicine,2 The Ohio State
University, Wooster, Ohio 44691
Received 10 April 2000/Returned for modification 10 May
2000/Accepted 10 July 2000
The major outer membrane protein (MOMP), a putative porin and a
multifunction surface protein of Campylobacter jejuni, may play an important role in the adaptation of the organism to various host environments. To begin to dissect the biological functions and
antigenic features of this protein, the gene (designated
cmp) encoding MOMP was identified and characterized from 22 strains of C. jejuni and one strain of C. coli.
It was shown that the single-copy cmp locus encoded a
protein with characteristics of bacterial outer membrane proteins.
Prediction from deduced amino acid sequences suggested that each MOMP
subunit consisted of 18
-strands connected by short periplasmic
turns and long irregular external loops. Alignment of the amino acid
sequences of MOMP from different strains indicated that there were
seven localized variable regions dispersed among highly conserved
sequences. The variable regions were located in the putative external
loop structures, while the predicted
-strands were formed by
conserved sequences. The sequence homology of cmp appeared
to reflect the phylogenetic proximity of C. jejuni strains,
since strains with identical cmp sequences had
indistinguishable or closely related macrorestriction fragment
patterns. Using recombinant MOMP and antibodies recognizing linear or
conformational epitopes of the protein, it was demonstrated that
the surface-exposed epitopes of MOMP were predominantly
conformational in nature. These findings are instrumental in the design
of MOMP-based diagnostic tools and vaccines.
*
Corresponding author. Mailing address: Food Animal
Health Research Program, Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Ave., Wooster, OH 44691. Phone: (330) 263-3747. Fax: (330) 263-3677. E-mail: zhang.234{at}osu.edu.
Infection and Immunity, October 2000, p. 5679-5689, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Zeng, X., Xu, F., Lin, J.
(2009). Molecular, Antigenic, and Functional Characteristics of Ferric Enterobactin Receptor CfrA in Campylobacter jejuni. Infect. Immun.
77: 5437-5448
[Abstract]
[Full Text]
-
Hobb, R. I., Fields, J. A., Burns, C. M., Thompson, S. A.
(2009). Evaluation of procedures for outer membrane isolation from Campylobacter jejuni. Microbiology
155: 979-988
[Abstract]
[Full Text]
-
Kumagai, Y., Huang, H., Rikihisa, Y.
(2008). Expression and Porin Activity of P28 and OMP-1F during Intracellular Ehrlichia chaffeensis Development. J. Bacteriol.
190: 3597-3605
[Abstract]
[Full Text]
-
Sahin, O., Plummer, P. J., Jordan, D. M., Sulaj, K., Pereira, S., Robbe-Austerman, S., Wang, L., Yaeger, M. J., Hoffman, L. J., Zhang, Q.
(2008). Emergence of a Tetracycline-Resistant Campylobacter jejuni Clone Associated with Outbreaks of Ovine Abortion in the United States. J. Clin. Microbiol.
46: 1663-1671
[Abstract]
[Full Text]
-
Qian, H., Pang, E., Du, Q., Chang, J., Dong, J., Toh, S. L., Ng, F. K., Tan, A. L., Kwang, J.
(2008). Production of a Monoclonal Antibody Specific for the Major Outer Membrane Protein of Campylobacter jejuni and Characterization of the Epitope. Appl. Environ. Microbiol.
74: 833-839
[Abstract]
[Full Text]
-
Sommerlad, S. M., Hendrixson, D. R.
(2007). Analysis of the Roles of FlgP and FlgQ in Flagellar Motility of Campylobacter jejuni. J. Bacteriol.
189: 179-186
[Abstract]
[Full Text]
-
Akiba, M., Lin, J., Barton, Y.-W., Zhang, Q.
(2006). Interaction of CmeABC and CmeDEF in conferring antimicrobial resistance and maintaining cell viability in Campylobacter jejuni. J Antimicrob Chemother
57: 52-60
[Abstract]
[Full Text]
-
Lin, J., Akiba, M., Sahin, O., Zhang, Q.
(2005). CmeR Functions as a Transcriptional Repressor for the Multidrug Efflux Pump CmeABC in Campylobacter jejuni. Antimicrob. Agents Chemother.
49: 1067-1075
[Abstract]
[Full Text]
-
Luo, N., Pereira, S., Sahin, O., Lin, J., Huang, S., Michel, L., Zhang, Q.
(2005). Enhanced in vivo fitness of fluoroquinolone-resistant Campylobacter jejuni in the absence of antibiotic selection pressure. Proc. Natl. Acad. Sci. USA
102: 541-546
[Abstract]
[Full Text]
-
Nikaido, H.
(2003). Molecular Basis of Bacterial Outer Membrane Permeability Revisited. Microbiol. Mol. Biol. Rev.
67: 593-656
[Abstract]
[Full Text]
-
Sahin, O., Luo, N., Huang, S., Zhang, Q.
(2003). Effect of Campylobacter-Specific Maternal Antibodies on Campylobacter jejuni Colonization in Young Chickens. Appl. Environ. Microbiol.
69: 5372-5379
[Abstract]
[Full Text]
-
Lin, J., Sahin, O., Michel, L. O., Zhang, Q.
(2003). Critical Role of Multidrug Efflux Pump CmeABC in Bile Resistance and In Vivo Colonization of Campylobacter jejuni. Infect. Immun.
71: 4250-4259
[Abstract]
[Full Text]
-
Luo, N., Sahin, O., Lin, J., Michel, L. O., Zhang, Q.
(2003). In Vivo Selection of Campylobacter Isolates with High Levels of Fluoroquinolone Resistance Associated with gyrA Mutations and the Function of the CmeABC Efflux Pump. Antimicrob. Agents Chemother.
47: 390-394
[Abstract]
[Full Text]
-
Varghees, S., Kiss, K., Frans, G., Braha, O., Samuel, J. E.
(2002). Cloning and Porin Activity of the Major Outer Membrane Protein P1 from Coxiella burnetii. Infect. Immun.
70: 6741-6750
[Abstract]
[Full Text]
-
Dedieu, L., Pages, J.-M., Bolla, J.-M.
(2002). Environmental Regulation of Campylobacter jejuni Major Outer Membrane Protein Porin Expression in Escherichia coli Monitored by Using Green Fluorescent Protein. Appl. Environ. Microbiol.
68: 4209-4215
[Abstract]
[Full Text]
-
Lin, J., Michel, L. O., Zhang, Q.
(2002). CmeABC Functions as a Multidrug Efflux System in Campylobacter jejuni. Antimicrob. Agents Chemother.
46: 2124-2131
[Abstract]
[Full Text]
-
(2002). Molecular typing of micro-organisms: at the centre of diagnostics, genomics and pathogenesis of infectious diseases?. J Med Microbiol
51: 7-10
[Full Text]
-
Sahin, O., Zhang, Q., Meitzler, J. C., Harr, B. S., Morishita, T. Y., Mohan, R.
(2001). Prevalence, Antigenic Specificity, and Bactericidal Activity of Poultry Anti-Campylobacter Maternal Antibodies. Appl. Environ. Microbiol.
67: 3951-3957
[Abstract]
[Full Text]