This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gosink, K. K.
Right arrow Articles by Masure, H. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gosink, K. K.
Right arrow Articles by Masure, H. R.

 Previous Article  |  Next Article 

Infection and Immunity, October 2000, p. 5690-5695, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Role of Novel Choline Binding Proteins in Virulence of Streptococcus pneumoniae

Khoosheh K. Gosink, Elizabeth R. Mann, Chris Guglielmo, Elaine I. Tuomanen,* and H. Robert Masure

Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38105

Received 13 March 2000/Returned for modification 16 May 2000/Accepted 5 July 2000

The choline binding proteins (CBPs) are a family of surface proteins noncovalently bound to the phosphorylcholine moiety of the cell wall of Streptococcus pneumoniae by a conserved choline binding domain. Six new members of this family were identified, and these six plus two recently described cell wall hydrolases, LytB and LytC, were characterized for their roles in virulence. CBP-deficient mutants were constructed and tested for adherence to eukaryotic cells, colonization of the rat nasopharynx, and ability to cause sepsis. Five CBP mutants, CbpD, CbpE, CbpG, LytB, and LytC, showed significantly reduced colonization of the nasopharynx. For CbpE and -G this was attributable to a decreased ability to adhere to human cells. CbpG, a putative serine protease, also played a role in sepsis, the first observation of a pneumococcal virulence determinant strongly operative both on the mucosal surface and in the bloodstream.


* Corresponding author. Mailing address: Department of Infectious Diseases, St. Jude Children's Research Hospital, 332 N. Lauderdale, Memphis, TN 38105. Phone and fax: (901) 495-3300. E-mail: elaine.tuomanen{at}stjude.org.


Infection and Immunity, October 2000, p. 5690-5695, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Holmlund, E., Quiambao, B., Ollgren, J., Jaakkola, T., Neyt, C., Poolman, J., Nohynek, H., Kayhty, H. (2009). Antibodies to Pneumococcal Proteins PhtD, CbpA, and LytC in Filipino Pregnant Women and Their Infants in Relation to Pneumococcal Carriage. CVI 16: 916-923 [Abstract] [Full Text]  
  • Munoz-Elias, E. J., Marcano, J., Camilli, A. (2008). Isolation of Streptococcus pneumoniae Biofilm Mutants and Their Characterization during Nasopharyngeal Colonization. Infect. Immun. 76: 5049-5061 [Abstract] [Full Text]  
  • Monterroso, B., Saiz, J. L., Garcia, P., Garcia, J. L., Menendez, M. (2008). Insights into the Structure-Function Relationships of Pneumococcal Cell Wall Lysozymes, LytC and Cpl-1. J. Biol. Chem. 283: 28618-28628 [Abstract] [Full Text]  
  • Gonzalez, A., Llull, D., Morales, M., Garcia, P., Garcia, E. (2008). Mutations in the tacF Gene of Clinical Strains and Laboratory Transformants of Streptococcus pneumoniae: Impact on Choline Auxotrophy and Growth Rate. J. Bacteriol. 190: 4129-4138 [Abstract] [Full Text]  
  • Rice, K. C., Bayles, K. W. (2008). Molecular Control of Bacterial Death and Lysis. Microbiol. Mol. Biol. Rev. 72: 85-109 [Abstract] [Full Text]  
  • Mahdi, L. K., Ogunniyi, A. D., LeMessurier, K. S., Paton, J. C. (2008). Pneumococcal Virulence Gene Expression and Host Cytokine Profiles during Pathogenesis of Invasive Disease. Infect. Immun. 76: 646-657 [Abstract] [Full Text]  
  • Attali, C., Frolet, C., Durmort, C., Offant, J., Vernet, T., Di Guilmi, A. M. (2008). Streptococcus pneumoniae Choline-Binding Protein E Interaction with Plasminogen/Plasmin Stimulates Migration across the Extracellular Matrix. Infect. Immun. 76: 466-476 [Abstract] [Full Text]  
  • Perez-Dorado, I., Campillo, N. E., Monterroso, B., Hesek, D., Lee, M., Paez, J. A., Garcia, P., Martinez-Ripoll, M., Garcia, J. L., Mobashery, S., Menendez, M., Hermoso, J. A. (2007). Elucidation of the Molecular Recognition of Bacterial Cell Wall by Modular Pneumococcal Phage Endolysin CPL-1. J. Biol. Chem. 282: 24990-24999 [Abstract] [Full Text]  
  • Saskova, L., Novakova, L., Basler, M., Branny, P. (2007). Eukaryotic-Type Serine/Threonine Protein Kinase StkP Is a Global Regulator of Gene Expression in Streptococcus pneumoniae. J. Bacteriol. 189: 4168-4179 [Abstract] [Full Text]  
  • Lanie, J. A., Ng, W.-L., Kazmierczak, K. M., Andrzejewski, T. M., Davidsen, T. M., Wayne, K. J., Tettelin, H., Glass, J. I., Winkler, M. E. (2007). Genome Sequence of Avery's Virulent Serotype 2 Strain D39 of Streptococcus pneumoniae and Comparison with That of Unencapsulated Laboratory Strain R6. J. Bacteriol. 189: 38-51 [Abstract] [Full Text]  
  • Moscoso, M., Garcia, E., Lopez, R. (2006). Biofilm Formation by Streptococcus pneumoniae: Role of Choline, Extracellular DNA, and Capsular Polysaccharide in Microbial Accretion. J. Bacteriol. 188: 7785-7795 [Abstract] [Full Text]  
  • Fillon, S., Soulis, K., Rajasekaran, S., Benedict-Hamilton, H., Radin, J. N., Orihuela, C. J., El Kasmi, K. C., Murti, G., Kaushal, D., Gaber, M. W., Weber, J. R., Murray, P. J., Tuomanen, E. I. (2006). Platelet-Activating Factor Receptor and Innate Immunity: Uptake of Gram-Positive Bacterial Cell Wall into Host Cells and Cell-Specific Pathophysiology. J. Immunol. 177: 6182-6191 [Abstract] [Full Text]  
  • Obert, C., Sublett, J., Kaushal, D., Hinojosa, E., Barton, T., Tuomanen, E. I., Orihuela, C. J. (2006). Identification of a Candidate Streptococcus pneumoniae Core Genome and Regions of Diversity Correlated with Invasive Pneumococcal Disease.. Infect. Immun. 74: 4766-4777 [Abstract] [Full Text]  
  • McCullers, J. A. (2006). Insights into the Interaction between Influenza Virus and Pneumococcus. Clin. Microbiol. Rev. 19: 571-582 [Abstract] [Full Text]  
  • Barocchi, M. A., Ries, J., Zogaj, X., Hemsley, C., Albiger, B., Kanth, A., Dahlberg, S., Fernebro, J., Moschioni, M., Masignani, V., Hultenby, K., Taddei, A. R., Beiter, K., Wartha, F., von Euler, A., Covacci, A., Holden, D. W., Normark, S., Rappuoli, R., Henriques-Normark, B. (2006). A pneumococcal pilus influences virulence and host inflammatory responses. Proc. Natl. Acad. Sci. USA 103: 2857-2862 [Abstract] [Full Text]  
  • Mann, B., Orihuela, C., Antikainen, J., Gao, G., Sublett, J., Korhonen, T. K., Tuomanen, E. (2006). Multifunctional Role of Choline Binding Protein G in Pneumococcal Pathogenesis. Infect. Immun. 74: 821-829 [Abstract] [Full Text]  
  • Bergmann, S., Hammerschmidt, S. (2006). Versatility of pneumococcal surface proteins. Microbiology 152: 295-303 [Abstract] [Full Text]  
  • Moscoso, M., Obregon, V., Lopez, R., Garcia, J. L., Garcia, E. (2005). Allelic Variation of Polymorphic Locus lytB, Encoding a Choline-Binding Protein, from Streptococci of the Mitis Group. Appl. Environ. Microbiol. 71: 8706-8713 [Abstract] [Full Text]  
  • Radin, J. N., Orihuela, C. J., Murti, G., Guglielmo, C., Murray, P. J., Tuomanen, E. I. (2005). {beta}-Arrestin 1 Participates in Platelet-Activating Factor Receptor-Mediated Endocytosis of Streptococcus pneumoniae. Infect. Immun. 73: 7827-7835 [Abstract] [Full Text]  
  • Moscoso, M., Lopez, E., Garcia, E., Lopez, R. (2005). Implications of Physiological Studies Based on Genomic Sequences: Streptococcus pneumoniae TIGR4 Synthesizes a Functional LytC Lysozyme. J. Bacteriol. 187: 6238-6241 [Abstract] [Full Text]  
  • Garau, G., Lemaire, D., Vernet, T., Dideberg, O., Di Guilmi, A. M. (2005). Crystal Structure of Phosphorylcholine Esterase Domain of the Virulence Factor Choline-binding Protein E from Streptococcus pneumoniae: NEW STRUCTURAL FEATURES AMONG THE METALLO-{beta}-LACTAMASE SUPERFAMILY. J. Biol. Chem. 280: 28591-28600 [Abstract] [Full Text]  
  • Kausmally, L., Johnsborg, O., Lunde, M., Knutsen, E., Havarstein, L. S. (2005). Choline-Binding Protein D (CbpD) in Streptococcus pneumoniae Is Essential for Competence-Induced Cell Lysis. J. Bacteriol. 187: 4338-4345 [Abstract] [Full Text]  
  • Guiral, S., Mitchell, T. J., Martin, B., Claverys, J.-P. (2005). From The Cover: Competence-programmed predation of noncompetent cells in the human pathogen Streptococcus pneumoniae: Genetic requirements. Proc. Natl. Acad. Sci. USA 102: 8710-8715 [Abstract] [Full Text]  
  • Fernandez-Tornero, C., Garcia, E., de Pascual-Teresa, B., Lopez, R., Gimenez-Gallego, G., Romero, A. (2005). Ofloxacin-like Antibiotics Inhibit Pneumococcal Cell Wall-degrading Virulence Factors. J. Biol. Chem. 280: 19948-19957 [Abstract] [Full Text]  
  • Segal, S., Pollard, A. J. (2005). Vaccines against bacterial meningitis. Br Med Bull 72: 65-81 [Abstract] [Full Text]  
  • Haas, W., Sublett, J., Kaushal, D., Tuomanen, E. I. (2004). Revising the Role of the Pneumococcal vex-vncRS Locus in Vancomycin Tolerance. J. Bacteriol. 186: 8463-8471 [Abstract] [Full Text]  
  • Orihuela, C. J., Radin, J. N., Sublett, J. E., Gao, G., Kaushal, D., Tuomanen, E. I. (2004). Microarray Analysis of Pneumococcal Gene Expression during Invasive Disease. Infect. Immun. 72: 5582-5596 [Abstract] [Full Text]  
  • Hamel, J., Charland, N., Pineau, I., Ouellet, C., Rioux, S., Martin, D., Brodeur, B. R. (2004). Prevention of Pneumococcal Disease in Mice Immunized with Conserved Surface-Accessible Proteins. Infect. Immun. 72: 2659-2670 [Abstract] [Full Text]  
  • Steinmoen, H., Teigen, A., Havarstein, L. S. (2003). Competence-Induced Cells of Streptococcus pneumoniae Lyse Competence-Deficient Cells of the Same Strain during Cocultivation. J. Bacteriol. 185: 7176-7183 [Abstract] [Full Text]  
  • Ren, B., Szalai, A. J., Thomas, O., Hollingshead, S. K., Briles, D. E. (2003). Both Family 1 and Family 2 PspA Proteins Can Inhibit Complement Deposition and Confer Virulence to a Capsular Serotype 3 Strain of Streptococcus pneumoniae. Infect. Immun. 71: 75-85 [Abstract] [Full Text]  
  • Martin, P. K., Bao, Y., Boyer, E., Winterberg, K. M., McDowell, L., Schmid, M. B., Buysse, J. M. (2002). Novel Locus Required for Expression of High-Level Macrolide-Lincosamide-Streptogramin B Resistance in Staphylococcus aureus. J. Bacteriol. 184: 5810-5813 [Abstract] [Full Text]  
  • Ogunniyi, A. D., Giammarinaro, P., Paton, J. C. (2002). The genes encoding virulence-associated proteins and the capsule of Streptococcus pneumoniae are upregulated and differentially expressed in vivo. Microbiology 148: 2045-2053 [Abstract] [Full Text]  
  • Steinmoen, H., Knutsen, E., Havarstein, L. S. (2002). Induction of natural competence in Streptococcus pneumoniae triggers lysis and DNA release from a subfraction of the cell population. Proc. Natl. Acad. Sci. USA 99: 7681-7686 [Abstract] [Full Text]  
  • Balachandran, P., Brooks-Walter, A., Virolainen-Julkunen, A., Hollingshead, S. K., Briles, D. E. (2002). Role of Pneumococcal Surface Protein C in Nasopharyngeal Carriage and Pneumonia and Its Ability To Elicit Protection against Carriage of Streptococcus pneumoniae. Infect. Immun. 70: 2526-2534 [Abstract] [Full Text]  
  • Swiatlo, E., Champlin, F. R., Holman, S. C., Wilson, W. W., Watt, J. M. (2002). Contribution of Choline-Binding Proteins to Cell Surface Properties of Streptococcus pneumoniae. Infect. Immun. 70: 412-415 [Abstract] [Full Text]  
  • Magee, A. D., Yother, J. (2001). Requirement for Capsule in Colonization by Streptococcus pneumoniae. Infect. Immun. 69: 3755-3761 [Abstract] [Full Text]