Role of Novel Choline Binding Proteins in Virulence of Streptococcus pneumoniae

doi:10.1128/IAI.68.10.5690-5695.2000

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Infection and Immunity, October 2000, p. 5690-5695, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Role of Novel Choline Binding Proteins in Virulence of Streptococcus pneumoniae

Khoosheh K. Gosink, Elizabeth R. Mann, Chris Guglielmo, Elaine I. Tuomanen,^* and H. Robert Masure

Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38105

Received 13 March 2000/Returned for modification 16 May 2000/Accepted 5 July 2000

The choline binding proteins (CBPs) are a family of surface proteins noncovalently bound to the phosphorylcholine moiety ofthe cell wall of Streptococcus pneumoniae by a conserved cholinebinding domain. Six new members of this family were identified,and these six plus two recently described cell wall hydrolases,LytB and LytC, were characterized for their roles in virulence.CBP-deficient mutants were constructed and tested for adherenceto eukaryotic cells, colonization of the rat nasopharynx, andability to cause sepsis. Five CBP mutants, CbpD, CbpE, CbpG, LytB,and LytC, showed significantly reduced colonization of the nasopharynx.For CbpE and -G this was attributable to a decreased ability toadhere to human cells. CbpG, a putative serine protease, alsoplayed a role in sepsis, the first observation of a pneumococcalvirulence determinant strongly operative both on the mucosal surfaceand in thebloodstream.

^* Corresponding author. Mailing address: Department of Infectious Diseases, St. Jude Children's Research Hospital, 332 N. Lauderdale,Memphis, TN 38105. Phone and fax: (901) 495-3300. E-mail: elaine.tuomanen{at}stjude.org.

Infection and Immunity, October 2000, p. 5690-5695, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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