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Infection and Immunity, October 2000, p. 5809-5815, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Vaccination of Cattle with Mycobacterium bovis Culture Filtrate Proteins and Interleukin-2 for Protection against Bovine Tuberculosis

D. Neil Wedlock,1,* Bridget Vesosky,2 Margot A. Skinner,1 Geoffrey W. de Lisle,1 Ian M. Orme,2 and Bryce M. Buddle1

AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand,1 and Mycobacteria Research Laboratories, Department of Microbiology, Colorado State University, Fort Collins, Colorado 805232

Received 10 April 2000/Returned for modification 15 May 2000/Accepted 3 July 2000

In this study vaccines prepared from culture filtrate proteins (CFP) of Mycobacterium bovis and interleukin-2 (IL-2) were tested in cattle for their capacity to stimulate immune responses and to protect against an intratracheal challenge with virulent M. bovis. Nine groups of cattle were vaccinated with combinations of different doses of CFP and bovine IL-2 mixed with a monophosphoryl lipid A (MPL) adjuvant. An additional group was vaccinated with M. bovis BCG. Immune responses in CFP-IL-2-vaccinated animals differed from those seen in BCG-vaccinated animals by inducing high antigen-specific antibody responses and low levels of gamma interferon and IL-2 released from purified protein derivative-stimulated whole-blood cultures. In a concurrent experiment, additional animals were added to the high-dose CFP-IL-2, MPL control, and BCG groups and these expanded groups of animals were challenged intratracheally with virulent M. bovis. Although the lung lesion scores were significantly lower for both the CFP-IL-2-and BCG-vaccinated groups compared to the MPL control group, the overall level of protection was greatest for the BCG-vaccinated animals. There were more animals with extrathoracic spread of disease in the CFP-IL-2 group than in the other groups. While vaccination of cattle with M. bovis CFP gave an encouraging reduction in tuberculous lesions and did not induce a delayed-type hypersensitivity response to PPD, future CFP vaccines must prevent any extrathoracic spread of disease.

* Corresponding author. Mailing address: AgResearch, Wallaceville Animal Research Centre, P.O. Box 40063, Upper Hutt, New Zealand. Phone: 64 4 9221593. Fax: 64 4 9221380. E-mail: wedlockn{at}agresearch.cri.nz.

Infection and Immunity, October 2000, p. 5809-5815, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Wedlock, D. N., Denis, M., Painter, G. F., Ainge, G. D., Vordermeier, H. M., Hewinson, R. G., Buddle, B. M. (2008). Enhanced Protection against Bovine Tuberculosis after Coadministration of Mycobacterium bovis BCG with a Mycobacterial Protein Vaccine-Adjuvant Combination but Not after Coadministration of Adjuvant Alone. CVI 15: 765-772 [Abstract] [Full Text]  
  • Wedlock, D. N., Denis, M., Skinner, M. A., Koach, J., de Lisle, G. W., Vordermeier, H. M., Hewinson, R. G., van Drunen Littel-van den Hurk, S., Babiuk, L. A., Hecker, R., Buddle, B. M. (2005). Vaccination of Cattle with a CpG Oligodeoxynucleotide-Formulated Mycobacterial Protein Vaccine and Mycobacterium bovis BCG Induces Levels of Protection against Bovine Tuberculosis Superior to Those Induced by Vaccination with BCG Alone. Infect. Immun. 73: 3540-3546 [Abstract] [Full Text]  
  • Buddle, B. M., Wedlock, D. N., Parlane, N. A., Corner, L. A. L., de Lisle, G. W., Skinner, M. A. (2003). Revaccination of Neonatal Calves with Mycobacterium bovis BCG Reduces the Level of Protection against Bovine Tuberculosis Induced by a Single Vaccination. Infect. Immun. 71: 6411-6419 [Abstract] [Full Text]  
  • Skinner, M. A., Buddle, B. M., Wedlock, D. N., Keen, D., de Lisle, G. W., Tascon, R. E., Candido Ferraz, J., Lowrie, D. B., Cockle, P. J., Vordermeier, H. M., Glyn Hewinson, R. (2003). A DNA Prime-Mycobacterium bovis BCG Boost Vaccination Strategy for Cattle Induces Protection against Bovine Tuberculosis. Infect. Immun. 71: 4901-4907 [Abstract] [Full Text]  


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